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Studies on Genetic Polymorphism of Beta-Lactoglobulin Gene in Nandidurg Goat

KrishiKosh

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Title Studies on Genetic Polymorphism of Beta-Lactoglobulin Gene in Nandidurg Goat
 
Creator Arshan Shaheed, A.
 
Contributor Nagaraja, C.S.
Jayashankar, M.R.
Girish Kumar, V
Veere Gowda, B.M.
 
Subject Studies on Genetic Polymorphism of Beta-Lactoglobulin Gene in Nandidurg Goat
 
Description PG. Thesis
A study was conducted with the objective of analyzing the genetic variation at the
β-lactoglobulin region of Nandidurg non-descriptive breed of goat by using PCR- RFLP
technique. Genomic DNA was isolated from blood samples of 85 animals. The genetic
diversity of caprine β-lactoglobulin locus was investigated by PCR- RFLP. A 426 bp
fragment of exon 7 to the 3‟ flanking region of the β-lactoglobulin gene was amplified.
The size of the amplified product was same in all the animals studied indicating
conservation of DNA at this locus. A restriction endonuclease SacII was used to detect
the genetic variation of the experimental unit in the β-lactoglobulin gene. β-LG/SacII
polymorphism was observed as three patterns which allowed the identification of two
alleles viz., A and B. The genotypic frequencies of AA, AB and BB were 0.35, 0.54 and
0.11 respectively. The allelic frequencies for A and B alleles were 0.62 and 0.38
respectively, among them allele A was predominant in studied population. The
population studied was in Hardy-Weinberg equilibrium. The sequence analysis indicated
that there was a high homology between the present result and the published caprine
β-lactoglobulin sequences. There was query coverage to the extent of 100 per cent. The
present study revealed the genetic variability at β-lactoglobulin gene locus in Nandidurg
goat. Further study is suggested to understand the association of this gene with milk
production traits.
 
Date 2016-11-14T15:41:28Z
2016-11-14T15:41:28Z
2013-09-15
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85449
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar