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Map Kinase Signaling In Ovine Coronary Vascular Smooth Muscle

KrishiKosh

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Title Map Kinase Signaling In Ovine Coronary Vascular Smooth Muscle
 
Creator Vijayakumar, C.
 
Contributor Mathuram, L.N.
Selvasubramanian, S.
Raj, G. Dhinakar
 
Subject Ovine coronary vascular smooth muscle
5 HT receptor
Muscarinic receptor
MAP kinase signaling
Curcumin
 
Description The present study was undertaken to identify the sensitivity of ovine coronary artery to
different vasoactive agents and involvement of the mitogen activated protein (MAP) kinase family of extracellular signal regulated kinase (ERK) 1 and 2 in agonist mediated vasoconstriction. The activity of curcumin in the ovine coronary artery was also studied.
Ovine coronary vascular smooth muscle was highly sensitive to alpha methyl serotonin, 5
hydroxytryptamine and acetylcholine and insensitive to phenylephrine and prostaglandin F2 alpha.
Alpha methyl serotonin and 5 hydroxytryptamine induced contraction in ovine coronary
vascular smooth muscle was completely abolished by ketanserin a specific 5 HT2 receptor
antagonist which indicated that contractile response mediation was through 5 HT2 receptors in the
ovine coronary artery.
Acetylcholine produced significant concentration related contractile response in
endothelium denuded ovine coronary vascular smooth muscle. The contractile response was
significantly inhibited by pre incubation of tissue with specific M1 and M3 receptor blocker,
pirenzepine and para Fluorohexahydro-sila-difenidol, respectively which revealed the existence of
M1 and M3 receptor in the ovine coronary artery.
5 hydroxytryptamine and acetylcholine induced vasoconstriction was measured in the
ovine coronary arterial ring in the presence or absence of MEK inhibitor. Thereafter the ERK1/2
phosphorylation in the ovine coronary vascular smooth muscle was quantified using western blot
analysis and ELISA. 5 hydroxytryptamine and acetylcholine induced a concentration dependent
vasoconstriction, which could be inhibited by the selective MEK inhibitor, U 0126. Further
confirmation with western blot analysis and ELISA revealed a significant increase in the ERK1/2
phosphorylation induced by 5 hydroxytryptamine and acetylcholine. These results indicate that
vasoconstriction induced by 5 hydroxytryptamine and acetylcholine is partly regulated by the ERKMAP
kinase pathway.
Curcumin produced significant relaxant effect on 5 hydroxytryptamine induced pre
contractile response in the endothelium denuded ovine coronary vascular smooth muscle.
 
Date 2016-05-30T15:52:44Z
2016-05-30T15:52:44Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66459
 
Language en
 
Format application/pdf
 
Publisher Tamil Nadu Veterinary and Animal Sciences University