Record Details

MARKER ASSISTED SELECTION FOR CONVERSION OF ELITE MAIZE INBREDS INTO HQPM LINES WITH HIGH LYSINE AND TRYPTOPHAN

KrishiKosh

View Archive Info
 
 
Field Value
 
Title MARKER ASSISTED SELECTION FOR CONVERSION OF ELITE MAIZE INBREDS INTO HQPM LINES WITH HIGH LYSINE AND TRYPTOPHAN
 
Creator KRISHNA MOTUKURI, S.R.
 
Contributor SOKKA REDDY, S
 
Subject maize, amino acids, selection, genetics, proteins, genes, planting, breeds (animals), germplasm, biological phenomena
 
Description Development of QPM (Quality Protein Maize) with high lysine and tryptophan
is foremost important task in maize breeding programme. Marker assisted selection is
the easiest way of developing QPM hybrids in short time. The present investigation
deals with identification of suitable donors for QPM hybrid development, Diversity
analysis was carried out among the QPM germplasm and attempts to conversion of
elite maize inbred lines (BML 6 and BML 7) into HQPM lines. One hundred and ten
lines were screened with three gene (opaque-2) specific markers (umc1066, phi 057
and phi 112). Among these lines CML 181 was identified as a potential donor which
showed good polymorphism with non QPM inbred lines BML 6 and BML 7, the
parental lines of DHM 117, the promising hybrid of the state.
Genetic diversity was analysed among the QPM germplasm lines with SSR
markers. A total of 151 alleles were identified from the 37 polymorphic SSR loci
among 63 QPM inbreds. The UPGMA cluster analysis based dendrogram grouped the
63 genotypes into 4 major clusters with a genetic similarity range of 66 per cent to 97
per cent. These diversified QPM inbreds can be used for hybridization programme.
Non QPM inbred lines BML 6 and BML 7 were crossed with QPM donor
CML 181 during kharif 2010. Foreground selection was carried out with gene specific
marker umc1066 in F1 population. Foreground selected plants of both the crosses were
backcrossed with respective recurrent parents BML 6 and BML 7 during rabi 2010-
11. Two hundred BC1F1 plants were screened with umc1066 for foreground selection.
Foreground selected plants were backcrossed with recurrent parents BML 6 and BML
7. BC2F1 population was screened with umc1066 for opaque-2 gene. The selected
plants were screened for foreground selection with amino acid modifiers.
Whole genome background selection was carried out with 752 SSR markers
for BML 6 and BML7. Foreground selected plants for both opaque-2 and amino acid
modifiers were screened for background selection. Recurrent parent genome (RPG)
was calculated for both the BC2F1 populations. Three plants were observed with RPG
90 – 93 per cent in BML 6 derived population. Two plants were observed with RPG
90 - 93 per cent in BML 7 derived back cross population. The plants showing more
than 80 % RPG were in turn back crossed with respective recurrent parents (BML 6
and BML 7). The programme will be continued till both the parental lines are
converted and high QPM hybrid is developed.
 
Date 2016-06-23T09:06:38Z
2016-06-23T09:06:38Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67764
 
Language en
 
Relation D9133;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY