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Dietary Manipulation Of Fat On Mitochondrial Decay And Its Implications On Ageing And Apoptosis

KrishiKosh

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Title Dietary Manipulation Of Fat On Mitochondrial Decay And Its Implications On Ageing And Apoptosis
 
Creator David, Corbon Godfrey, I.
 
Contributor Leela, V.
Thangavel, A.
Mathuram, L.N.
Vairamuthu, S.
 
Subject Calorie restriction
Dietary fat
Coconut oil
Corn oil
Mitochondria
Oxidative stress
Antioxidant status
Apoptosis
Ageing
Heart
Brain
 
Description An investigation was carried out to study the effect of dietary
manipulation of fat and calorie restriction (CR) on the production of ROS
in relation to activity of mitochondrial electron transport complexes and
its implications on apoptosis in ageing heart and brain.
Eighteen months old male Wistar Albino rats were fed on coconut
and corn oil based diets ad libitium or at 40 per cent CR up to 24 (short
term) or 30 months (long term) of age. At the end of feeding trial rats
were sacrificed. Mitochondria from heart and brain were isolated for the
estimation of membrane fatty acid composition, H2O2 and OXPHOS
enzyme activity. Mitochondrial inner membrane surface area was
measured stereologically from electron micrographs. Antioxidant enzyme
status, markers of oxidative stress measured as TBARS and protein
carbonyls and DNA fragmentation were measured in both the tissues.
Myocardial collagen and serum lipid profile were also estimated to
correlate the effect of dietary fat and CR on retarding ageing.
Both short and long term feeding of linoleic acid low coconut oil
and linoleic acid rich corn oil based diets with CR increased oleic (18:1)
linoleic acid (18:2) levels respectively in the heart and brain
mitochondrial membranes. Reinforcement of 18:1 levels in membranes
increased inner membrane surface area, membrane potential and
reduced H2O2 production in rats fed on coconut oil based diet with CR
compared to corn oil diet. The oxidative enzyme activities did not show
appreciable age related variation in rats fed on coconut oil and corn oil
based diets.
Although, short term feeding of both coconut oil and corn oil based
diets with CR effectively minimized age related changes in heart and
brain, on the long run coconut oil based diet with CR was found effective
to reduce oxidative damage to lipids and proteins but not DNA through
enhanced antioxidant status. It is concluded that coconut oil based diets
with CR reduces age related changes in heart and brain through
reinforcement of membrane fatty acids, improved membrane integrity
and decreased oxidative stress.
 
Date 2016-05-30T15:32:14Z
2016-05-30T15:32:14Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66458
 
Language en
 
Format application/pdf
 
Publisher Tamil Nadu Veterinary and Animal Sciences University