ICAR Research Data Repository for Knowledge Management
Comparison of Diagnostic tests for Paratuberculosis
KrishiKosh
View Archive Info| Field | Value | |
| Title |
Comparison of Diagnostic tests for Paratuberculosis
IVJ_V89_V06_Pg15-17 |
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| Creator |
Kumar, O.R. Vinodh
Faris, D. Senthil, N.R. Gunaseelan, L. |
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| Description |
Sixty three paratuberculosis suspected dairy animals were taken for this study and subjected to single intradermal test, faecal culture and IS900 nested PCR. Thirty three animals were found positive by single intradermal test, 40 samples were positive by faecal culture and 58 samples were positive by nested PCR. 40 faecal samples out of 63 were positive by IS900 primary PCR assay for Mycobacterium avium subsp. Paratuberculosis (Map) and 23 faecal samples, which gave negative results in the primary PCR, were subjected to secondary PCR assay. Of the 23 samples, 18 samples gave positive results in the IS900 nested PCR (nPCR), giving better sensitivity than single amplification PCR. The chi-square analysis showed a highly significant difference between the tests (P
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| Date |
2016-07-04T14:52:34Z
2016-07-04T14:52:34Z 2012-06 |
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| Type |
Article
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/68419
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| Language |
en
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| Format |
application/pdf
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| Publisher |
Indian Veterinary Journal
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