BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF SIDEROPHORES PRODUCED BY FLUORESCENT PSEUDOMONAS SPECIES AND THEIR ROLE IN THE REPLANT PROBLEM OF APPLE
KrishiKosh
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Title |
BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF SIDEROPHORES PRODUCED BY FLUORESCENT PSEUDOMONAS SPECIES AND THEIR ROLE IN THE REPLANT PROBLEM OF APPLE
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Creator |
SHARMA, SAPNA
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Contributor |
KAUR, MOHINDER
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Subject |
bacteria, planting, iron, productivity, biological development, concentrates, sampling, fungi, diseases, apples
apple orchard, fluorescent Pseudomonas |
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Description |
ABSTRACT The present study was aimed at the isolation of fluorescent Pseudomonas strains from normal and replant site of apple orchard. The fluorescent Pseudomonas sp are plant beneficial rhizobacteria with all the direct and indirect plant growth promoting activities like siderophores, phosphate solubilization, antifungal and production of HCN; ammonia; plant growth regulators (auxins, cytokinins and gibberellins) and lytic enzymes. Siderophore is an important secondary metabolite of fluorescent Pseudomonas as it solubilize the unavailable form of iron in soil and make it available to plant which is an important micronutrient required by the plants. Pyoverdin type of siderophore was identified from the Pseudomonas aeruginosa strain An-1-Rho, An-4-Rho and An-4-U strains with molecular weight 1111.70, 1215 and 727.1 respectively after purification and characterization. Ferric ion biosensor developed from purified siderophore an easy way to measure iron concentration in different soil samples and assay is linear, highly specific, robust and accurate as proved by repeated experimentation. Antifungal activities of Pseudomonas help in suppression of plant pathogenic fungus in soil and these may be the reasons of better establishment of apple seedling treated with fluorescent Pseudomonas strain and results in increase in Pseudomonas count and decreased total rhizobial count. Therefore these strains can be exploited for the management of replant problem of apple. These selected genotypically and phenotypically characterized strains of Pseudomonas sp. which were found to be potent and can have great importance in the field of agriculture, especially in the treatment of apple plants in replant problem area |
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Date |
2016-06-23T14:14:22Z
2016-06-23T14:14:22Z 2012 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/67855
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Language |
en
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Format |
application/pdf
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