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Molecular Characterization and Serodiagnosis of Vascular Pathogens Affecting Tomato

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Title Molecular Characterization and Serodiagnosis of Vascular Pathogens Affecting Tomato
 
Creator Sumangala Koulagi
 
Contributor S. Lingaraju
 
Subject Plant Pathology
 
Description Soil borne pathogens affecting tomato (Fusarium oxysporum f. sp. lycopersici,
Ralstonia solanacearum and Meloidogyne incognita) were collected from different
tomato growing regions of Karnataka. F. o. f. sp. lycopersici isolates, viz. Fol-1, Fol-
4, Fol-6 Fol-9, Fol-11, Fol-13, Fol-15 and Fol-21 showed abundant aerial mycelium
and sporulation with maximum colony diameter (75 to 90.0 mm): These were highly
virulent. Among twenty four isolates of R. solanacearum, Rs-1, Rs-4, Rs-7, Rs-8, Rs-
9, Rs-12, Rs-16, Rs-19, Rs-21, Rs-22, Rs-23 and Rs-24 isolates were highly virulent.
RAPD analysis of F. o. f. sp. lycopersici revealed five major clusters. Maximum
genetic similarity (73 %) was between Gubbi (Fol-5) and Doddaballapur isolates (Fol-
4), whereas least genetic similarity was observed between Chintamani (Fol-17) and
Garag (Fol-2) isolates. The similarity co-efficient of R. solanacearum isolates ranged
from 0.19 to 0.61. Maximum genetic diversity of 61 per cent was between Hosalli
(Rs-7) and Doddaballapur (Rs-9) isolates whereas least similarity (0.19 per cent) was
observed between Kolar (Rs-22) and Garag (Rs-2) isolates. PCR performed with the
primer combination of Forward primer (5 -TGTATAAGTTTAATCGTTTTAACGA-
3 ) and 18s reverse primer (5 -GTATGTACCAACTATTTAGTAGGT- 3 ) produced
only the single expected fragment of 1.3 kb for all isolates of M. incognita. DASELISA
was more sensitive: it was precise enough to detect the F. o. f. sp. lycopersici
antigen up to 51200 dilutions. Purified antigen of F. o. f. sp. lycopersici
concentrations of 3.2 μg and 50 conidia/well could be detected by DAC –ELISA
technique: The technique was capable of detecting R. solanacearum at 103 cells/ml;
collected isolates of R. solanacearum showed positive reaction using DAC- ELISA
techinique. This technique was also able to detect R. solanacearum directly from soil
and plant sample infected with the bacterium. Four isolates M. incognita collected
from different regions showed positive reaction by DAC- ELISA assay.
 
Date 2016-07-25T11:20:49Z
2016-07-25T11:20:49Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69718
 
Format application/pdf
 
Publisher UAS Dharwad