MYCOFLORA INVOLVED IN POST HARVEST FRUIT ROT OF PAPAYA (Carica papaya L.) AND ITS MANAGEMENT
KrishiKosh
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Title |
MYCOFLORA INVOLVED IN POST HARVEST FRUIT ROT OF PAPAYA (Carica papaya L.) AND ITS MANAGEMENT
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Creator |
PATEL, JITENDRA B.
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Contributor |
MEHTA, B.P.
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Subject |
fruits, diseases, papayas, fungi, biological phenomena, application methods, pathogens, markets, irrigation, harvesting
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Description |
Papaya (Carica papaya L.) is one of the most important commercially grown fruit crops in tropical and sub-tropical regions of the world. India is one of the leading country in production of papaya, Gujarat is major contributor in production. It is also grown extensively in south Gujarat. During the survey, occurrence of fruit rot disease of papaya was noticed in a serious proportion inflic ting heavy losses in vegetable markets of Navsari and neighboring cities in popular variety Taiwan Red Lady. Considering the seriousness of the problem, the present investigation was carried out to find out exact cause and suitable measures for the management of fruit rot disease of papaya . Market survey of four major markets of the south Gujarat was carried out in two consecutive years i.e. 2011 and 2012 for prevalence of fruit rot of papaya disease which revealed average maximum per cent disease incidence (PDI) in Sardar market, Surat as 15.13 % followed by Dudhiya Talav market, (14.40%) Navsari, Sardar market, Billimora (13.80%) and Morarji Market, Navsari (12.26%). Out of five months observations, the maximum fruit rot (Av.19.50%) incidence was recorded in the month of April. It was also found that maximum rotted fruits yie lded Colletotrichum sp. (44.14%) followed by other five rotting pathogens. Isolations from rotted fruits collected from different markets revealed association of different six fungal pathogens which based on morphological features, cultural characteristics and as per report received from Indian Type Culture Collection (ITCC), New Delhi, were identified as Colletotrichum gloeosporioides (ITCC No.: 8173.11), Fusarium solani (ITCC No.: 8176.11), Alternaria alternata (ITCC No.: 8174.11), Rhizopus stolonifer (ITCC No.: 8178.11),, Aspergillus flavus (ITCC No.: 8175.11), and Aspergillus niger (ITCC No.: 8177.11). The pathogenicity was proved by artificial inoculation viz., cork borer, knife and pin prick injury methods and all the isolates gave higher per cent infection of fruit rots. Inoculation through various avenues revealed that the fungus (C. gloeosporioides) can enter the fruits through natural opening of stem end without pedicel and with pedicel as well rarely enters through lateral end of the fruit and caused lower infection whereas, heavy infection was found through the knife injury wounds. Effect of different temperatures and humidity were studied on the fruit rot (C. gloeosporioides ) of papaya which revealed that the inoculated fruits incubated at 300C temperature cause faster symptom initiation (1.2 days) as well as took less time (5.0 days) for 75 per cent rotting as compared to other incubation temperatures. In case of incubation at 95 per cent humidity, early symptom initiation (1.0 day) was observed as well as quick rotting of the fruits (4.2 days for 75 % rotting) occurred as compared to lower humidity incubation . The effect of the papaya fruit latex on different six fruit rotting fungal pathogens was studied by poisoned food technique using 15 per cent fruit latex in the medium (PDA) which showed highest growth inhibition (73.70 %) in A. flavus, followed by A. niger, C. gloeosporioides , A. alternata, R. stolonifer and F. solani. Studies regarding nutritional content changes in fruits due to infection of various fungal pathogens were undertaken showed considerable decreased in the total soluble solids, total sugar and ascorbic acid content of the infected fruits. Whereas, the reduc ing, nonreducing sugar and titerable acidity of the fruits were as compared to healthy ones. Six varieties of papaya were screened against fruit rot pathogen. Among them the variety Washington was found resistant whereas, Solo showed moderately resistant. However, Sunrise and Taiwan Red Lady were found moderately susceptible . Madhubindu was found susceptible and the desi/local cultivar was found highly susceptible to fruit rot disease. Out of six different wrapping materials were tested wrapping fruits with news paper showed the least disease incidence (8.33 %) followed by wrapping with butter paper, tissue paper, brown paper bag, alluminium foil and polyethylene bag were found effective against fruit rotting pathogens. Studies on hot water dip treatment (HWT) against the disease revealed that HWT at 500C for twenty minutes recorded significantly lowest disease incidence (6.00 %) and delayed the fruit rot (72 hrs) disease infection. Six phytoextracts of different plant species were screened at 10 per cent concentrations. Among them, leaf extract of deshi babul proved significantly more effective in checking the fruit rot (4.67 %) as well as taken maximum time (72 hrs) for fruit rotting initiation . Out of six oils tested, castor oil recorded minimum (10.33 PDI) with late symptoms initiation (72 hrs), followed by palm oil, cotton oil, soyabean oil, mustard oil and ground nut oil. Pre inoculated fruits were dipped in cell suspension of seven different isolates of antagonists. Pseudomonas aeruginosa Pa R (Rambhas) isolate recorded minimum PDI (15.67%) as well as maximum duration (72 hrs) for the initiation of symptoms followed by rest of the fungal and bacterial antagonists tested. Six different fungicides were tested in fie ld condition as pre - harvest sprayings for their effectiveness against post-harvest rot (C. gloeosporioides ) at three different locations viz., Navsari, Gandevi and Nizar. Two sprays of combi-product of carbendazim (12%) + mancozeb (63%) at 14 days interval on entire fruit column @ 1 g / l (11 .00 %) and mancozeb @ 1 g / l (12.11 %) showed least PDI o f post harvest fruit rot followed by carbendazim @ 1g / l which was at par with combination product of Strobilurin + Metiram @ 1 g / l and hexaconazole @ 1 ml / l. the highest per cent disease incidence of post harvest fruit rot was observed in kresoxim methyl @ 1ml / l in field conditions. |
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Date |
2016-05-03T11:06:01Z
2016-05-03T11:06:01Z 2013-05 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/65744
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Language |
en
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Format |
application/pdf
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Publisher |
Navsari Agricultural University, Navsari
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