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Varietal identification and seed vigour assessment in cotton (Gossypium spp.)

KrishiKosh

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Title Varietal identification and seed vigour assessment in cotton (Gossypium spp.)
 
Creator Harish, S.
 
Contributor Verma, S.S.
 
Subject sowing, cotton, developmental stages, genetics, proteins, planting, germinability, electrophoresis, diseases, land resources
 
Description The present investigation “Varietal identification and seed vigour assessment of cotton (Gossypium spp.)” was
comprised of 22 cotton varieties were studied for characterization based on morphological, chemical, biochemical and
molecular markers. On the basis of morphological characters, results revealed that maximum variation was observed
among the morphological characters studied for hypocotyl pigmentation, leaf colour, leaf hairiness, leaf appearance,
leaf nectaries, leaf petiole pigmentation, leaf shape, plant stem hairiness, plant stem pigmentation, plant height (cm),
bract type, time to 50% flowering, flower petal colour, flower petal spot, flower stigma, anther filament colouration,
flower pollen colour, boll bearing habit, boll colour, boll shape (longitudinal section), boll surface, boll prominence of
tip, weight of seed cotton/boll (g), seed fuzz, seed index (g), ginning % and fibre colour proved to be useful and stable
diagnostic characters which could classify the varieties based on the phenotype traits.
Chemical tests such as phenol, ferrous sulphate, peroxidase, potassium hydroxide and sodium hydroxide test were
used to categorize different cotton varieties. Phenol and ferrous sulphate test did not show any variation among the
varieties studied. Based on the colour of the peroxidase solution, the varieties were grouped as colourless and light red
colour. Based on the colour of the potassium hydroxide test, the varieties were grouped as light yellow, yellow, light
red, red and dark red colour and the cotton varieties were grouped as light red, red and dark red colour to sodium
hydroxide test. Seedling response to gibberellic acid and 2,4-D soak test was found useful in differentiation of cotton
varieties. Among the varieties, H-1300 observed maximum responsive (75.36 % coleoptile length increase over
control) to GA3 test and H-1226 observed less responsive (23.37 % coleoptile length decrease over control) to 2, 4-D
test. The varieties were also subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of
total soluble seed proteins. The results revealed that SDS-PAGE of seed proteins resolve total of 22 bands which were
present in different position with different intensity and varieties were classified based on presence or absence of
protein bands. The Gossypium hirsutum varieties were identified with the presence of band No.5 where as, Gossypium
arboreum varieties were identified by the presence of band No.15 and the band number No.7, 14, 17, 18, 21 and 22
were present typically in all the cotton varieties. Furthermore, the SSR markers were able to reveal genetic variation
within the species. Out of the 40 microsatellite markers, 12 markers produced a total of 32 polymorphic bands. The
similarity coefficient obtained in SSR analysis ranged from 0.51 to 0.93 among the varieties and the dendrogram
revealed two distinct clusters, first consisting of 16 varieties belonging to G. hirsutum and second with 6 varieties
belonging to G. arboreum species, respectively. The similarity coefficient between the two species was 0.52.
All the 22 varieties were subjected to various viability and vigour tests and the observations were recorded on seed
weight, seed germination, seedling length, seedling dry weight, seedling vigour index-I, seedling vigour index- II,
accelerated ageing test, tetrazolium test, seedling establishment, pH exudate test, electrical conductivity, field
emergence index and mean germination time. The variety CISA-614 recorded maximum germination (85.67 %) and
minimum electrical conductivity (0.336) whereas, MCU-12 recorded maximum seedling dry weight (529.00 mg),
seedling vigour index-I (2495) and seedling vigour index-II (43544). The variety AKA-7 recorded maximum field
emergence index (15.55) and minimum mean germination time (2.86 days).The correlation study among field and
laboratory parameters revealed that standard germination was found significantly and positively correlated with
accelerating ageing, tetrazolium test, pH exudate test, and vigour index-I. The electrical conductivity test was found
to be negatively correlated with all the parameters.
 
Date 2016-02-03T11:47:38Z
2016-02-03T11:47:38Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/64144
 
Language en
 
Format application/pdf
 
Publisher CCSHAU