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Identification and characterization of novel anti-inflammatory proteins/peptides from salivary glands of hyalomma ticks

KrishiKosh

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Title Identification and characterization of novel anti-inflammatory proteins/peptides from salivary glands of hyalomma ticks
 
Creator Ghosh, Mayukh
 
Contributor Sangwan, Nirmal
 
Subject Proteomics, Mass Spectrometery, Electrophoresis, Bioinformatics, Antiinflammatory, ELISA
 
Description Tick-borne diseases pose a significant threat to the livestock production system
especially in developing countries. Immunoprophylactic control has emerged as the most
promising strategy for controlling tick infestations. Identification of key molecules in tick
metabolic pathways and evaluation of their vaccine potential has become prime focus in tick
biology research. Exploration of tick sialome has revealed plethora of pharmacologically
active biomolecules including several anti-inflammatory candidates, immunomodulators,
enzyme inhibitors, anticoagulants etc. However, in the era of therapeutic peptides, the tick
derived bioactive molecules carry enormous potential to be used in different chronic
inflammatory diseases, autoimmune disorders and even in cancer. Therefore, the present
study focused on identification of novel anti-inflammatory proteins from salivary gland
extract of Hyalomma ticks, the vector of tropical theileriosis. Mass spectrometry based
proteomic approach (MALDI-MS/MS) following one and two-dimensional electrophoresis
was employed for characterization of the salivary anti-inflammatory protein.
Phosphatidylethanolamine-binding protein (PEBP) was the sole anti-inflammatory protein
identified in tick saliva having MW 19.1 kDa with pI of 9.1 and was of secretory in nature. In
this protein, several phosphorylation and glycosylation sites were identified which indicated
its possible role in regulation of cellular and metabolic pathways. It was also observed that the
protein contained PEBP domain which was of evolutionary conserved in nature and was
having potential regulatory role in NF-κβ mediated inflammatory response, fibroblast growth
factor (FGF) and epidermal growth factor (EGF) mediated mitogenesis. The protein
suppressed TNF-α release from bovine peripheral blood mononuclear cells and thus justified
its potential role as anti-inflammatory candidate. The multifaceted potential of the molecule in
immunoprophylactic and therapeutic applications will certainly propel further research to
develop it as novel candidate vaccine or therapeutics
 
Date 2016-08-17T10:46:15Z
2016-08-17T10:46:15Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/72686
 
Language en
 
Format application/pdf
 
Publisher LUVAS