Record Details

BIOCHEMICAL AND MOLECULAR BASIS OF INNATE AND Pseudomonas fluorescens INDUCED STEM ROT TOLERANCE IN GROUNDNUT (Arachis hypogaea L.)

KrishiKosh

View Archive Info
 
 
Field Value
 
Title BIOCHEMICAL AND MOLECULAR BASIS OF INNATE AND Pseudomonas fluorescens INDUCED STEM ROT TOLERANCE IN GROUNDNUT (Arachis hypogaea L.)
 
Creator SUJIT KUMAR BISHI
 
Contributor Vakharia D.N.
 
Subject STEM ROT TOLERANCE IN GROUNDNUT
BIOCHEMISTRY
 
Description Key words: 16s rRNA, tyloses, Ca-oxalate, gene expression, pgip
Groundnut (Arachis hypogaea L.), is an important legume crop and India is
one among the leading producers of groundnut worldwide. Sclerotium rolfsii is the
causal organism of stem rot in groundnut. Biological control of S. rolfsii using
antagonistic fluorescent Pseudomonas is a potential management tool. Four groundnut
genotypes such as CS19, GG16, GG20 and TG37A were selected to investigate the
biochemical, molecular and histopathological basis of their varying degree of
tolerance to stem rot disease. Eleven fluorescent Pseudomonas strains were isolated
from different groundnut growing locations of Saurasthra region of Gujarat. To study
the induced tolerance a potential antagonistic fluorescent Pseudomonas isolate (SKPf
5) belonging to species Pseudomonas aeruginosa was isolated and used as a treatment
in the study. Scanning Electron Microscopy of stem tissues at five days post S. rolfsii
infection revealed the formation of tyloses only in the tolerant genotype CS19 and the
accumulations of Ca-oxalate crystals like structures only in the most susceptible genotype
TG37A. Infection of S. rolfsii reported differential induction of antioxidative enzymes
like Superoxide Dismutase, Catalase, Ascorbate Peroxidase and Glutathione
Reductase; and pathogenesis related proteins such as chitinase and ß-1, 3-glucanases.
In general a rapid, early and stronger induction was observed for the tolerant as
compared to the susceptible genotypes. Application of SKPf 5 as seed-priming and
root inoculants reduced the disease incidence by promoting the overall growth of the
plants and activating the innate defense system. Stem rot infection resulted decrease
in ascorbic acid content and increase in oxalic acid content in all genotypes however
the trend was higher in susceptible genotypes. The proteomic study using twodimensional
gel electrophoresis indicated change in protein expression as a response
to fungal infection. The differential expression pattern of PR protein genes such as PR2,
PR4, PR5, and PR10 revealed an instant and stronger expression of those genes upon the
fungal stress as compare to the weaker and/or delayed or no induction in the susceptible
ones. A partial putative PGIP protein encoding gene was isolated and reported for the first
time in groundnut (acc. no. KP844637). PGIP gene expression showed its higher induction
in tolerant compared to susceptible genotypes as a response to S. rolfsii infection suggesting
its possible role in stem rot stress tolerance in groundnut.
 
Date 2016-09-28T08:39:53Z
2016-09-28T08:39:53Z
2015-10
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/79214
 
Language en
 
Format application/pdf