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DUS characterization on morphological, biochemical and molecular basis in Brassica juncea L.Czern & Coss and Brassica carinata A. Braun

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Title DUS characterization on morphological, biochemical and molecular basis in Brassica juncea L.Czern & Coss and Brassica carinata A. Braun
 
Creator Nitin Kumar
 
Contributor Pant, D.P.
 
Subject indian mustard, brassica juncea, brassica carinata, seasonal behaviour, plant morphology, characterization, molecular markers
 
Description Thesis-PhD
The present study was carried out with 26 mustard varieties including 16 Indian mustard and 10 Ethiopian mustard varieties over two rabi seasons of 2011-12 and 2012-13.Data were recorded for 24 morphological DUS descriptors (8 visually assessed and 16measurable). In Indian mustard among the eight visually assessed DUS descriptors, only one was monomorphic, three were dimorphic and four were polymorphic, while in Ethiopian mustard four were monomorphic, only one was dimorphic and three were polymorphic. In
Indian mustard out of 16 measurable characteristics, three were monomorphic, eight were dimorphic and five were polymorphic. In Ethiopian mustard seven characteristics were monomorphic and dimorphic respectively and two were polymorphic. COYD analysis with respect to 16 measurable traits indicated that all the varieties were absolutely distinct from each other in case of Indian mustard and Ethiopian mustard. COYD analysis was supported with MJRA analysis in which the slope of the MJRA curve and regression probability was calculated, which indicated that characteristics under study were not completely independent rather they are interacting with each other as well as with the environment. COYU analysis revealed that all the varieties were more or less uniform for measurable characteristics in ecotypes. Statistical analysis for stability, comparing respective values of PCV and GCV based on morphological data showed same magnitude for twelve measurable characteristics in Indian mustard and nine characteristics in Ethiopian mustard. This indicated that
environmental influence was less for the expression of these characters. High heritability coupled with high to moderate genetic advance for most of the measurable characteristics indicating their consistency and stability over the years and their utility in varietal characterization. On the basis of grouping characteristics none of the Indian mustard and Ethiopian mustard varieties could establish distinctiveness by unique morphological profiles. However, all the varieties of Indian mustard and Ethiopian mustard were discriminated when all the morphological descriptors were considered. SDS-PAGE protein profiling of mustard varieties revealed moderate level of polymorphism. Only NDRE-4 and PRB-2006-5 variety of Indian mustard could establish
distinctiveness when clustering was done using the UPGMA method on the basis of banding pattern scoring. Molecular marker analysis was conducted with SSRs. Twenty SSR primers produced unique banding pattern in three Indian mustard varieties Kranti, Krishna and PRB-2006-5 and two Ethiopian mustard varieties Kiran Early and PBC-2009-3. Thus, morpho-physiological DUS descriptors were found reliable and reproducible for establishing the distinctiveness of the varieties under study. Sometimes morphological DUS descriptors alone
may not be sufficient for establishing distinctiveness of varieties, but when supplemented with biochemical and molecular markers varieties could be discriminated. The varieties and those strains satisfying the DUS criteria could be register to utilize with PPV and FR Authority for breeders and farmers rights and also in crop improvement programme.
 
Date 2016-06-14T15:27:51Z
2016-06-14T15:27:51Z
2014-02
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67379
 
Language en
 
Format application/pdf
 
Publisher G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)