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IDENTIFICATION OF QTLS FOR SEEDLING ROOT TRAITS IN RICE (Oryza sativa L.) GENOTYPES

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Title IDENTIFICATION OF QTLS FOR SEEDLING ROOT TRAITS IN RICE (Oryza sativa L.) GENOTYPES
 
Creator SUNEETHA MADHURI, M
 
Contributor KESHAVULU, K
 
Subject QTLS, SEEDLING, ROOT, TRAITS, RICE, GENOTYPES
 
Description The present investigation was undertaken with an objective to assess seedling
vigour traits in rice genotypes, identify SSR markers for seedling root related traits and
study association of genomic SSRs with seedling root traits and vigour. The material for
investigation comprised of 47 rice varieties, comprising high, medium and low vigour
genotype. The experiment was laid out in a Completely Randomized Design with three
replications at the Department of Seed Science and Technology, College of
Agriculture, Acharya N. G. Ranga Agricultural University and molecular analysis was
carried out at Directorate of Rice Research, ICAR, Rajendranagar, Hyderabad.
Significant differences were observed for all the seedling vigour traits viz.,
hundred seed weight, electrical conductivity, germination, shoot length, root length,
seedling length, root shoot ratio, seedling fresh weight, seedling dry weight and vigour
indices. Three genotypes BPT 5204, Kalinga III and Sampada had low seed weight, while
the rest of the genotypes have medium to high seed weight. Of all the genotypes, Bharani
Deep, IR 28, Pant Dhan16, Shabagyadhan, Suraksha, Vandana, VL Dhan82 and VL
Dhan 208 showed low EC values. Highest germination percentage was recorded in
Akshadhan, RP 2421, IR 28, Sahbhagyadhan, Sasyasree, Swarna, Vandana, VL Dhan 208
and Moroberekan.
High mean germination time was observed in Heera, Krishnahamsa, RP2421 and
Vandana. Bharani Deep, Dinesh, Narendra 118, Sahbhagidhan, Sasyasree and VL Dhan
221. High shoot length was observed in Dinesh, Heera, Jaldidhan, Narendra118, PNR 381,
Sabita, Vandana, and VL Dhan 221. Similarly, highest root shoot ratio was found in
Adithya, BPT 5204, Govind, NDR 97, Pant Dhan 16, Sahbhagidhan, Sasyasree, Sampada
and Swarna. Highest seedling length was observed in Bharani Deep, Dinesh, Sabita,
Sahhbagidhan, Narendra118, and VL Dhan 221.
Among all the genotypes, Akshadhan, Dinesh, Jaldidhan, Moroberekan, Nagina 22,
Suraksha and VL Dhan 208 recorded highest fresh shoot weight and Adithya, NDR 97,
Sasyasree, Swarna and Vikas recorded high fresh root weight. Fresh seedling weight was
showed higher in Akshadhan, Dinesh, Jaldidhan, Moroberekan, Nagina 22, Suraksha and
VL Dhan 208. Dinesh, Jaldidhan, Moroberekan, Nagina 22, Suraksha and VL Dhan 208
recorded high seedling dry weight. Highest seed vigour index I was found in Bharani
Deep, Dinesh, Sabita, Sahbhagidhan, Sasyasree, VL Dhan 208 and VL Dhan 221, where as
high SVI II was noted in Dinesh, Jaldidhan, Moroberekan, Nagina 22, Nipponbare,
Suraksha, VL Dhan 208 and VL Dhan 221.
Of all physiological seedling traits root shoot ratio, root fresh weight, seedling dry
weight and seed vigour indices showed high GCV per cent while root and shoot length,
root shoot ratio, root fresh weight, seedling dry weight and seedling vigour indices
recorded high PCV per cent. Among all the characters, highest heritability was recorded
for seedling fresh weight. Based on vigour index trait, eight genotypes were high in seed
vigour indices viz., Dinesh, Jaldidhan, Moroberekan, Nagina 22, Nipponbare, Suraksha,
VL Dhan 208 and VL Dhan 221.
The correlation studies revealed positive and significant association among all
seedling vigour related traits, except for electrical conductivity and root shoot ratio. SVI II
showed high positively correlated with germination, root and shoot length, seedling
length, fresh root weight, fresh shoot weight, seedling fresh weight, seedling dry weight
and SVI I. The normal distribution for root length, seedling length, root fresh weight and
shoot fresh weight and skewed distribution for electrical conductivity, final count, mean
germination, SVI I and SVI II was observed. High heritability coupled with high GAM
was observed for SVI II.
A total of 30 SSR markers analyzed for 15 QTLs in 47 genotypes, 16 markers
showed polymorphism. Among these markers, highest phenotypic and genotypic
correlation for seedling vigour traits was exhibited by RM351 and RM44. From the
15 QTLs selected 11 QTLs were found to be present in different genotypes. Some
genotypes showed multiple QTLs viz., Moroberekan has nine QTLs, Nipponbare has six
QTLs, VL Dhan 208 has five QTLs, DRR 38 has four QTLs. Three QTLs were present in
Pantdhan16, Tulasi and Vandana. Two QTLs were present in Sampada, Anjali,
Vivekadhan 62, Swarna, Dinesh. Final evaluation of high vigourous genotypes identified
with phenotypic data and root QTLs by controlled deterioration test showed that Suraksha
was the best genotype with high seed longevity period.
The present study provided an overview of the phenotypic varience of the rice
genotypes for seed vigour traits and their genetic diversity for root traits. There was large
range of similar values for some root traits in related cultivars as microsatellites provide
greater confidence for the assessment of polymorphism. The information about genetic
diversity of these cultivars will be very useful for proper selection of parents and rice
breeding programs especially for gene mapping and eventually for the application of
marker assisted selection (MAS).
 
Date 2016-09-08T10:43:43Z
2016-09-08T10:43:43Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/75976
 
Language en
 
Relation D9668;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD