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Molecular Characterization of Mineral Phosphate Solubilization In Serratia marcescens AND Methylobacterium sp.

KrishiKosh

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Title Molecular Characterization of Mineral Phosphate Solubilization In Serratia marcescens AND Methylobacterium sp.
 
Creator Archana Kumari
 
Contributor P.U. Krishnaraj
 
Subject Plant Biotechnology
 
Description The major goal of the present investigation was to study the mechanism of
mineral phosphate solubilization in Serratia marcescens and Methylobacterium sp.
All the 157 isolates of S. marcesens and 73 isolates of pink pigmented facultative
methylotrophs were screened for their mineral phosphate solubilization phenotype on
solid media viz., Modified Sperber’s and TCP agar media. Eight most potent isolates
along with one reference strain Serratia marcescens ER2 were further screened for
pH drop and Pi release in NBRIP-BPB and TCP broth. Both pH drops and Pi release
were highly correlated upto 15 days. AUDS-151 and PPFM87 were the most potent
‘P’ solubilizing isolates. Gluconic acid was detected in the culture supernatants of
these isolates through thin layer chromatographic technique. Effect of buffering and
phosphate stress on MPS phenotype of these isolates were observed by external
supply of tris buffer and K2HPO4 to the MSM agar medium. It indicates the metabolic
control of mineral phosphate solubilization by external factors which either resist the
change in pH or alters the gene expression. Tn5 mutants were generated from the
most potent P solubilizing isolate AUDS-151 by biological (Tn5) mutagenesis.
Mutants failed to show MPS activity even after 5 DAI. Strainal identity of AUDS-151
and PPFM-87 was confirmed as S. marcescens and Methylobacterium mesophilicum
respectively through 16S rDNA sequencing. All the pqq operon gene (s) were
amplified from AUDS-151 and PPFM-87 and the most conserved pqqE gene was
cloned into E. coli DH5a from both the isolates. Both the clones pAMK101 and
pASK101 were sequenced and showed 97 per cent homology with the
Methylobacterium sp. (CPOOOO367) and 94 per cent homology with Serratia
marcescens (DQ868536), respectively.
 
Date 2016-11-12T13:07:50Z
2016-11-12T13:07:50Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85217
 
Format application/pdf
 
Publisher UAS, Dharwad