Record Details

CHARACTERIZATION OF POTENTIALLY ACTIVE NEW PROBIOTIC STRAINS ISOLATED FROM DIFFERENT SOURCES AND TO STUDY THEIR PROSPECTS AS NUTRACEUTICAL AGENTS

KrishiKosh

View Archive Info
 
 
Field Value
 
Title CHARACTERIZATION OF POTENTIALLY ACTIVE NEW PROBIOTIC STRAINS ISOLATED FROM DIFFERENT SOURCES AND TO STUDY THEIR PROSPECTS AS NUTRACEUTICAL AGENTS
 
Creator GUPTA, ANUPAMA
 
Contributor SHARMA, NIVEDITA
 
Subject bacteria, biological phenomena, acidity, organic acids, productivity, antibiotics, fermentation, pathogens, poultry equipment, organic acid salts
probiotic lactic acid bacteria
 
Description ABSTRACT
The present investigation was carried out to isolate most efficient potential probiotic lactic acid bacteria
from rare and unexplored food sources, their screening, identification, safety assessment, evaluation of probiotic
attributes, metabolic fingerprinting as well the development of novel nutraceutical products. In total 27 bacterial
isolates were obtained from fermented/non fermented food sources. Out of all, 6 bacterial isolates were screened
by bit/disc as well as well diffusion method on the basis of their wide inhibitory spectrum against tested
pathogens. Isolates C-1, LB-CC, LB-WC, Ch-1, Ch-2 and 107 were selected for further studies having widest
antagonistic potential, which were isolated from kodo millet flour, lasoda bari, chuli and salori, respectively that
are traditional food products of Himachal Pradesh. Isolates C-1, LB-CC and LB-WC were identifies as
Pediococcus pentosaceus, Ch-1 as Enterococcus faecium, Ch-2 as Pediococcus acidilactici while 107 identified
as Pediococcus damnosus by 16S rRNA gene technique and registered in NCBI under accession no.
KM251461, KM251460, KJ489415, KJ541885, KJ541886 and KJ577588, respectively. Safety assessment of
isolates was done by evaluating antibiotic susceptibility, haemolytic, DNase and gelatinase activities. All the
isolates exhibited 80-100% antibiotic sensitivity, non-heamolytic, non-DNase and non-gelatinase activities,
thereby proving their safe status. These screened isolates were further examined for their probiotic potential viz.
acidity tolerance, bile tolerance, simulated gastrointestinal transit, auto-, co-aggregation, hydrophobicity,
adhesion to gastric as well as mammalian cell lines, competitive exclusion of pathogens, antimicrobial potential,
exopolysaccharides and β-galactosidase production, compatibility and cumulative probiotic potential. All the six
screened isolates were found to be highly acidity tolerant strains with 93.65-99.41 % survival rate at pH 3 for 3
h. These six isolates were able to resist high bile salt concentration i.e. 2.0% with 93.12-98.22 % survival rate
for 8 h. Resistance to gastrointestinal transit as found to be in a range from 43.09-90.12 %. All the six isolates
exhibited good autoaggregation capacity i.e. greater than 40% after 5 h and showed string hydrophobicity
towards xylene i.e. > 40%. All the isolates were able to adhere to gastric mucin with 14.7-49.5% adhesion and
all showed adhesion potential to mammalian epithelial cell lines. These screened probiotic isolates showed
broad and strong inhibitory spectrum against both gram positive as well as gram negative pathogenic bacteria
through secretion of antimicrobial metabolites viz. lactic acid (7.18-8.46 mg/l), H2O2 (0.24-0.38g/l) and
bacteriocins (77.33-100.0 AU/ml). Metabolic fingerprinting was done to elucidate the total metabolic profile of
isolates and P. pentosaceus LB-CC and P. acidilactici Ch-2 were being first lactic acid bacteria to be reported
for the production of squalene with anticancerous properties. The entire screened six isolates were highly
qualified with cumulative score of 95.83% and are being used to prepare probiotic nutraceutical products with
high antioxidant content viz. novel cereal based probiotic acidulant, probiotic enriched mango fortified
shrikhand and probiotic enriched pulse fortified yogurt and are successfully accepted in their sensory evaluation.
Hence, this study affirms the use of P. pentosaceus C-1, P. pentosaceus LB-CC, P. pentosaceus LB-WC, E.
faecium Ch-1, P. acidilactici Ch-2 and P. damnosus 107 in the development of new functional foods and
nutraceutical preparations to impart betterment to the public health as these six strains isolated in the present
study have been proved safe as well as highly effective probiotic candidates.
 
Date 2016-06-14T15:09:31Z
2016-06-14T15:09:31Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67375
 
Language en
 
Format application/pdf