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VARIABI STUDIES OF Fusarium oxysporum f. sp. ciceri (PADWICK) CAUSING WILT OF CHICKPEA (Cicer arietinum L.)

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Title VARIABI STUDIES OF Fusarium oxysporum f. sp. ciceri (PADWICK) CAUSING WILT OF CHICKPEA (Cicer arietinum L.)
 
Creator H R, CHAITHRA
 
Contributor M, SAIFULLA
 
Subject grain, rice, zinc, planting, genes, genotypes, polishing, biological phenomena, genetics, selection
 
Description Chickpea is one of the major grain legume crop widely grown in the Indian sub
continent, which accounts for almost 75 per cent of world’s production. Wilt caused by
Fusarium oxysporum f. sp. ciceri is the most serious disease causing 10-15 % each year.
Keeping this in view, twelve F. o. f. sp. ciceri isolates were isolated from infected
samples collected across different states of India. The cultural and morphological
characters viz., pigmentation, mycelial colour, growth pattern, length, width, number of
septations of microconidia and macroconidia, diameter of chlamydospores, shape, colour,
number of spores were considered to assess the variation. Out of 144 mycelial
combinations, 119 combinations showed compatible and 25 combinations were identified
as mycelial non-compatible. Among twelve isolates, MPFOC-9 showed highest radial
growth and was selected for nutritional and physiological studies. Glucose (455.41 mg &
84.87 mm) and Peptone (408.03 mg & 87.19 mm) recorded highest mycelial growth on
liquid and solid media. Maximum mycelial growth was recorded on liquid and solid
medium at pH 6.5 (535.30 mg and 88.33 mm) and temperature 300C (391.31 mg & 89.6
mm). Among 10 RAPD primers used to study genetic variation, nine primers showed 100
%, whereas OPA-12 showed 87.5 % polymorphism among 12 isolates and similarity
ranged from 43 to 76 %. Among 12 isolates, MPFOC-9 caused cent per cent wilt
incidence on chickpea host differentials. Different entries screened for wilt, 14 entries
showed resistant reactions under field condition and also recorded lowest ‘AUDPC’ and
‘r’ values.
 
Date 2016-10-25T13:05:27Z
2016-10-25T13:05:27Z
2014-07-08
 
Type Thesis
 
Identifier TH-10774
http://krishikosh.egranth.ac.in/handle/1/81797
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore