ICAR Research Data Repository for Knowledge Management
Characterization and immunological properties of mycorrhizal glycoprotein glomalin
KrishiKosh
View Archive Info| Field | Value | |
| Title |
Characterization and immunological properties of mycorrhizal glycoprotein glomalin
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| Creator |
Gupta, Sheetanshu
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| Contributor |
Mall, Rakesh
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| Subject |
characterization, immunology, mycorrhizas, glycoproteins, glomalin, organic fertilizers, organic farming, sustainability, pesticides, environmental protection
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| Description |
Thesis-PhD
Role of biofertilizers and biopesticides are being critical for sustainable agriculture especially in the production of green food. Arbuscular mycorrhizal symbiosis plays a significant role in plant growth enhancement through higher nutrient uptake and in soil stabilization, carbon sequestration through the production of unique and specific protein: glomalin. However, the role of glomalin in soil borne fungal suppression was not confirmed. Therefore, suppression of soilborne plant pathogens Sclerotium rolfsii and Fusarium oxysporum was investigated using glomalin extracted from the soil used for raising the maize plants inoculated with different arbuscular mycorrhizal fungi (AMF). Glomalin estimated from soil inoculated with G.coronatum contributed 14.27 mg/g of soil followed by G.intraradicis and G.mosseae, 10.64 and 10.20 mg/g of soil respectively. Molecular weight of glomalin was found 60kDa in SDS-PAGE after silver staining. Glomalin was immunogenic and the titre value of glomalin extracted from soils inoculated with different AMF species were 1:200 estimated by ELISA. In vitro testing of suppression of Sclerotium rolfsii and Fusarium oxysporum by glomalin at concentration 0.8 mg glomalin/petriplate showed maximum inhibition than 0.4mg/petriplate. G.coronatum showed 69.03% and 21.56% growth inhibition of S.rolfsii followed by G.intraradicis 29.80% and 12.30% and G.mosseae 20.10% and 6.17% at 0.8mg/Petriplate and 0.4mg/Petriplate of glomalin respectively.Percent inhibition of F. oxysporum was 69.47 % and 19.77 % treated with glomalin extracted from G. Coronatum followed by 35.3 % and 11.63 % treated with glomalin extracted from G. intraradices and 27.83 % and 10.37 % treated with glomalin extracted from G. mosseae at concentration 0.8 mg glomalin /petriplate and 0.4mg/petriplate respectively. Glomalin shows good suppression of wilt disease caused by Sclerotium rolfsii and Fusarium oxysporum under in vivo experiment along with AMF spores and hyphae whereas alone AMF did not show any suppression of wilt disease in tomato plants.There was no effect in production of glomalin protein by AMF during degradation of pesticides. All mycorrhizal AMF species shows same amount of pesticide degraded during production of glomalin. Concern for pesticide contamination in the environment it is great importance in which pesticides are degraded through AMF. |
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| Date |
2016-09-08T15:14:40Z
2016-09-08T15:14:40Z 2011-01 |
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| Type |
Thesis
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/76128
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| Language |
en
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| Format |
application/pdf
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| Publisher |
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
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