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Studies on Bacterial Blight of Cowpea Caused By Xanthomonas axonopodis pv. Vignicola (Burkh.) Vauterin et al.

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Title Studies on Bacterial Blight of Cowpea Caused By Xanthomonas axonopodis pv. Vignicola (Burkh.) Vauterin et al.
 
Creator Nandini R.
 
Contributor Shripad Kulkarni
 
Subject Plant pathology
 
Description Cowpea bacterial blight caused by Xanthomonas axonopodis pv. vignicola has
become major constraint in all the cowpea growing regions of northern Karnataka. Survey
data revealed that the highest disease severity (14.32 PDI) was observed in Belgaum district
followed by Gadag and Dharwad districts. Lowest disease severity (9.58 PDI) was observed
in Haveri district.
Detection of pathogen using different selective and semi-selective media revealed
NSCAA as best medium for isolating the pathogen from seeds with maximum number of
bacterial colonies(132×105 cfu/ml) followed by SIBU agar medium (126 × 105 cfu/ml). Van
vuurde et al. method opined that diseased seeds of susceptible variety C-152 yielded more
number of bacterial colonies (35×102) compared to the diseased seeds of resistant variety
DCS 47-1 which yielded 2×102 colonies.
Screening of varieties/gerplasm lines against bacterial blight of cowpea revealed DCS
47-1 and RC-101 varieties as resistant and C-152 × GL (F1) as highly susceptible. In vitro
evaluation of chemicals indicated that, Streptocycline (0.05%) + Copper oxy chloride (0.3%)
exhibited superior efficacy followed by Streptocycline (0.1%) and Copper oxy chloride
(0.3%) alone. Among botanicals garlic and soapnut extracts at 10 % concentration were
found inhibitory. Among bioagents, Pseudomonas fluorescens (isolate 1) was superior in
inhibiting growth of bacteria followed by Bacillus subtilis. In vivo studies indicated that
Streptocycline (0.05%) + COC (0.3%) and Streptocycline (0.05%) alone found effective in
reducing the disease severity of 89.07 and 81.12 per cent respectively. Hot water treatment
conducted in roll paper towel method and pot culture revealed that 520 C for 10 min was
found effective. Integrated management under field conditions revealed hot water treatment +
Seed treatment with Pseudomonas fluorescens (0.5%) + Foliar spray of Pseudomonas
fluorescens at 25 days and Streptocycline + COC (0.05 + 0.3%) spray at 45 days was
effective (9.25 PDI and yield 8.03 q/ha).
 
Date 2016-11-21T19:13:54Z
2016-11-21T19:13:54Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/86850
 
Format application/pdf
 
Publisher UAS, Dharwad