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Phenotyping and microsatellite marker analysis of F2 and backcross populations derived from the crosses between aerobic and Basmati rice varieties

KrishiKosh

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Title Phenotyping and microsatellite marker analysis of F2 and backcross populations derived from the crosses between aerobic and Basmati rice varieties
 
Creator Rajesh
 
Contributor Jain, R.K.
 
Subject rice, planting, vegetative propagation, yields, irrigation, alleles, grain, selection, developmental stages, biological phenomena
 
Description Increasing water scarcity has threatened the cultivation of lowland Basmati varieties in northern states of India including Haryana. Marker assisted selection can greatly improve the efficacy of Basmati rice breeding programs. In the present investigation, experiments were conducted to evaluate two F2 populations and BC1F1 plants derived from crosses between Basmati (Improved Basmati 370, IB370) and aerobic rice varieties (MAS25 and MAS-ARB25), for various physio-morphological and root traits under aerobic conditions in field and nethouse. Both the segregating F2 populations displayed large variation for grain yield (3.9-40.9 and 2-43.6 g), root length (22-50 and 31-65 cm), root dry biomass (0.7-9.3 and 0.7-11.8 g), root thickness (17.4-30.4 and 10.2-32 mm) and grain length/breadth ratio (3.4-5.2 and 3.5-5.5), respectively. Phenotypic correlation analysis showed that root length was positively correlated (r=0.496) with root thickness in IB370 × MAS25 F2 population. Grain yield per plant showed significant positive correlation (r=0.250) with root thickness in IB370 × MAS-ARB25 F2 population. A DNA fingerprint database of two segregating F2 populations, IB370 × MAS25 and IB370 × MAS-ARB25 was prepared using 61 and 58 polymorphic SSR markers, respectively, distributed on the entire genome of rice. The distribution of IB370 and MAS25/MAS-ARB25 specific alleles among IB370 × MAS25 and IB370 × MAS-ARB25 derived F2 plants were determined and SSR markers data was used to identify QTL for various agronomic and root traits. Composite Interval Mapping (CIM) analysis by WinQTL cartographer 2.5 revealed a total of 13 QTL (six for root traits and seven for agronomical traits) on chromosome 1, 2, 3, 8 and 10 and a total of seven QTL (four for root traits and three for agronomical traits) on chromosomes 2, 4 and 5 in IB370 × MAS25 and IB370 × MAS-ARB25 derived F2 populations, respectively. Twenty promising F2 plants each from both the two crosses were selected on the basis of aroma, root length, L/B ratio and grain yield/ plant for further progeny analysis. The putative QTL identified in the two F2 populations were also monitored in the selected promising F2 plants; these QTL were present in homozygous or heterozygous state in high frequencies. DNA fingerprint database of 13 rice varieties was prepared using 129 polymorphic SSR markers, NTSYS-pc analysis and two-dimensional PCA scaling exhibited that 13 rice genotypes were interspersed in the two groups. Group I consisted of aerobic and indica, while group II had Basmati rice genotypes. Similarity coefficient data showed that Basmati rice variety is almost equally distant from aerobic indica (0.584) and lowland indica (0.549) rice genotypes.
 
Date 2016-02-10T12:00:36Z
2016-02-10T12:00:36Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/64270
 
Language en
 
Format application/pdf
 
Publisher CCSHAU