Record Details

Fertility In Bitches Following Intravaginal And Intrauterine Insemination With Fresh And Frozen Semen

KrishiKosh

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Title Fertility In Bitches Following Intravaginal And Intrauterine Insemination With Fresh And Frozen Semen
 
Creator Sathiamoorthy, T
 
Contributor Asokan, S.A.
Veerapandian, C
Kalatharan, J
Nambi, A.P.
 
Subject Fertility
Artificial insemination
Bitches
 
Description This study was conducted to compare the conception rate and litter size
in bitches inseminated following intravaginal and intrauterine route with fresh
and frozen semen and to study the effect of commercial semen extender on
post thaw characters of dog semen. To study the efficacy of vaginoscopy to
predict fertile insemination time in bitches was also taken as one of the
objectives. Sixty bitches were randomly divided into six groups viz., Group I,
II, III, IV, V and VI 10 each. The time of insemination was based on
vaginoscopy in group I to V and on vaginal exfoliative cytology in group VI
bitches.
Semen was collected from seven male dogs of different breeds,
evaluated and used for cryopreservation either with Tris or Tes-tris extender.Group I and II bitches were subjected to intravaginal insemination while
Group III and IV bitches were subjected to intrauterine insemination using
fresh and frozen semen, respectively. Group V and VI bitches were
inseminated intravaginally using frozen semen supplemented with prostatic
fluid and with fresh semen using vaginal exfoliative cytology to determine time
of insemination, respectively. The conception rate obtained in this study was
50, 20, 50, 30, 30 and 40 per cent in group I, II, III, IV, V and VI bitches
respectively. The mean post thaw motility was significantly higher for semen
cryopreserved with Tris when compared to Tes-tris extender. The percentage
of mean abnormality was significantly lower in semen cryopreserved with Tris
when compared to Tes-tris extender. The mean percentage of plasma
membrane integrity was significantly higher for Tris when compared to Tes-
tris extender. Hence, it is concluded from this study that the conception rates
were similar for both intravaginal and intrauterine insemination with fresh
semen and higher conception rates were obtained when frozen semen was
deposited intrauterine rather than intravaginal. Semen cryopreserved with Tris
extender was found to gave better post thaw characters compared to Tes-tris
extender. Vaginoscopy was found to be effective in assessing the optimum
insemination time compared to vaginal exfoliative cytology. Addition of
prostatic fluid with frozen semen had significant influence on increasing the
conception rate with intravaginal insemination.
 
Date 2016-05-27T16:36:23Z
2016-05-27T16:36:23Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66409
 
Language en
 
Format application/pdf
 
Publisher Tamil Nadu Veterinary and Animal Sciences University