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Status of Synthetic Pyrethroid Susceptibility / Resistance in Rhipicephalus (Boophilus) Microplus Ticks of South India using Allele-specific Polymerase Chain Reaction

KrishiKosh

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Title Status of Synthetic Pyrethroid Susceptibility / Resistance in Rhipicephalus (Boophilus) Microplus Ticks of South India using Allele-specific Polymerase Chain Reaction
JVP_2012_26(2)_174-175
 
Creator Mathivathani, C.
Basith, S. Abdul
Latha, B.R.
Raj, G. Dhinakar
Sreekumar, C.
 
Subject Rhipicephalus (Boophilus) microplus
Synthetic pyrethroids
AS–PCR
Sodium channel gene
Carboxyl esterase gene
 
Description Rhipicephalus (Boophilus) microplus ticks with a previous history of acaricidal treatment
with synthetic pyrethroids (SP) such as cypermethrin, deltamethrin and flumethrin were
randomly collected from cattle of different pockets of southern states of India namely Tamil Nadu, Kerala, Karnataka, Andhra Pradesh and Puducherry from cattle. DNA extracted from pooled adult R. microplus ticks from each state was subjected to allele specific polymerase chain reaction (AS–PCR) to detect point mutation in sodium channel gene and a second AS–PCR was standardized to detect single nucleotide polymorphism in carboxyl esterase gene of R. microplus ticks. Mutation could not be detected either in sodium channel or carboxylesterase gene of R. microplus indicate that these ticks are susceptible to SP.
 
Date 2016-07-01T10:57:14Z
2016-07-01T10:57:14Z
2012
 
Type Article
 
Identifier http://krishikosh.egranth.ac.in/handle/1/68346
 
Language en
 
Format application/pdf
 
Publisher Journal of Veterinary Parasitology