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MOLECULAR ANALYSIS OF TRANSGENIC GROUNDNUT FOR DROUGHT TOLERANCE GENES

KrishiKosh

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Title MOLECULAR ANALYSIS OF TRANSGENIC GROUNDNUT FOR DROUGHT TOLERANCE GENES
 
Creator NEELIMA, S
 
Contributor MANORAMA, K
 
Subject MOLECULAR, ANALYSIS, TRANSGENIC, GROUNDNUT, DROUGHT, TOLERANCE, GENES
 
Description Groundnut (Arachis hypogaea L.) is an important legume cash crop whose crop
productivity and yield stability are affected by abiotic stresses such as drought. Drought
is a complex phenomenon. Research in transgenic crops may offer new means to improve
agriculture particularly in dry areas, as genes specifically involved in response to drought
have been identified (Lieu et al., 2000). However, a major challenge of transgenic
research besides obtaining transgenic material is to understand the responses of plants to
their external environment and identify new targets for improving stress tolerance.
Transgenic groundnut plants (rd29A: DREB1A) tolerant to drought, were initially
evaluated for the expression of npt II and DREB 1 A genes through PCR and RT-PCR.
The plants positive for npt II gene were subjected to water stress conditions using a
typical dry-down procedure (Ritchie 1980; Sinclair and Ludlow, 1986; Ray and Sinclair,
1997) in P2 level containment greenhouse. To understand the molecular mechanism and
differential gene expression under water stress conditions, a highly sensitive technique
namely DDRT-PCR (Differential display Reverse transcription-Polymerase chain
reaction), which facilitates identification and isolation of differentially expressed genes,
has been employed in the present study. Differential display studies were carried out
using 20 RT-PCR positive plants for DREB 1A gene. Twenty drought stress-responsive
partial cDNAs were initially identified by comparing expression profiles between water
stressed transgenic and non-transgenic plants by means of differential display. Among
these 9 were newly expressed (induced), 5 were up-regulated and 6 were down-regulated.
Interestingly, the sequence analysis of 8 differentially expressed clones identified
that the EST function of 4 cDNA clones was similar to that of stress related genes. Two
were similar to that of cold stress, one to that of water stress and one to abiotic stress. The
function of the remaining four clones was not known. This indicates that many of the
abiotic stress responsive pathways may be interlinked. For the clones identified to be
differentially expressed in response to drought, their stress-regulated expression and
function needs to be confirmed and investigated further. The transcripts identified in this
study also have great potential as molecular markers.
 
Date 2016-08-22T13:01:56Z
2016-08-22T13:01:56Z
2005
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/73273
 
Language en
 
Relation D7514;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD