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Indentification of molecular marker for interogression of mungbean mosaic virus (MYMV) resistant in mungbean (Vigna radiataL.)

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Title Indentification of molecular marker for interogression of mungbean mosaic virus (MYMV) resistant in mungbean (Vigna radiataL.)
 
Creator Kalaria, Rishee Kishorbhai
 
Contributor Mahatma, Lalit
 
Subject diseases, genes, planting, crossing over, rapd, selection, polymorphism, land resources, germplasm, dna
 
Description Twenty six germplas m lines were screened at the hot spot of Pulse Research Station (PRS), Navsari for t he finding resistant source of MYMV. Four germplasm lines viz. , NAUMR1, NAUMR2, NAUMR3 and MEHA showed highly resistant reaction against YMD. NAUMR1, NAUMR2 and
NAUMR3 were found late maturing, tall and produced few pods only. MEHA is MYMV resistant mungbean cultivar developed and released from IIPR, Kanpur in 2004, h owever, is of s mall size. Out of 200 RAPD markers, only fou rteen markers showed variable degree of polymorphism. Maximu m 90% polymorphism was observed in OPG-5, OPJ-18 and OPM-20 whereas minimum polymorphism (28.57 per cent) wa s in OPH-9. Overall polymorphis m was found to be 70.17 p er cent. Fourteen markers on five genotypes generated 126 ba nds out of which 90 were polymorphic band. OPG-5, OPJ-18 an d OPM-20 were proved to be the best markers for the s tudy of polymorphism as it produced 28, 35, 28 amplicons respectively. In OPG-5 a band of size ~850bp was ab sent in
susceptible cultivar GM4 while present in all the r esistant lines. In the marker OPJ-18, total 6 unique band o f approximately 250bp, 300bp, 450bp, 500bp, 850bp and 1250bp size were present in all resistant lines while abse nt in susceptible one. Similarly in the marker OPM-20 3 e xclusive
band 350bp, 400bp and 600bp size appeared in all re sistant lines whereas absent in susceptible GM4. The dendro gram obtained with range of similarity coefficient 0.41 to 0.79 clearly indicated two distinct main clusters A and B. Cluster A included susceptible GM4 and cluster B showed the a ll
resistant lines viz., NAUMR1, NAUMR2, NAUMR3 and MEHA. Cluster B bifurcated in to two subclusters, B1 and B2 with similarity coefficient of approx. 0.72. Subcluster B1 comprises MEHA whereas subcluster B2 contained rema ining three resistant lines. Diversity in all the resista nt and
susceptible were observed by the ISSR markers also. Out o f the seventeen RGA marker co mbinations, four markers amplified DNA from the resistant, however, could no t amplify any DNA fragment fro m the susceptible. On sequencin g all these fragment were found 98 % similarity with each other. Similarly in the case of other RGA fragments showed similarity with Vigna radiata viral resistance candidate. In F1 all the plants showed resistant phenotype and also the presence of specific band of ~450bp in molecular an alysis with the RGA markers which was absent in the suscep tible cultivar GM-4. The same followed phenotypic as well as genotypic segregation ratio of 3 (Resistant):1(Susc eptible) in F2. Interestingly in BC1F 1 , 10 plants were observed and the
1:1 ratio of phenotype and genotype was observed. D ata suggested that the resistance is governed by single do minant gene. Looking at the phenotypic, genotypic and agro nomic
characteristics, MEHA was found superior among all the resistant sources. Therefore MEHA is further propos ed for the introgression of resistant gene through breeding pr ogramme.
Use of the cultivated variety in breeding as donor is always preferred over uncharacterized germplasm as to redu ce linkage drag. Among four markers, RGA pair-2 (VRMYMVRGA-2) is
reco mmended to check the inheritance of gene in suc ceeding generations.
 
Date 2016-05-03T09:42:26Z
2016-05-03T09:42:26Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65733
 
Language en
 
Format application/pdf
 
Publisher NAU