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Evaluation of Diagnostic Tests in Rabies and Studies on Cytokine Expression in Mice

KrishiKosh

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Title Evaluation of Diagnostic Tests in Rabies and Studies on Cytokine Expression in Mice
 
Creator Sathish Mundas
 
Contributor Suguna Rao
Satyanarayana, M.L.
Yathiraj, S.
Byregowda, S.M.
Patil, S.S.
 
Subject ---
 
Description Ph.D. Thesis
A total of 114 rabies suspected brain samples collected from rabies suspected
cases submitted to Department of Veterinary Pathology, Veterinary College, Bangalore
and Veterinary hospitals in and around Bangalore. The brain samples were subjected to
Seller‟s, FAT, dRIT and RT-PCR to arrive at confirmatory diagnosis of rabies. Sixty four
of 114 samples were positive by Seller‟s staining, seventy seven samples turned positive
by both FAT and dRIT; where as 81 rabies suspected brain samples were found positive
by RT-PCR. In the diagnosis of rabies, RT-PCR test was found to have the highest
sensitivity followed by FAT, dRIT and Seller‟s staining. dRIT can be used for the
screening of the suspected samples in field level because diagnosis can be made by using
light microscope and its accuracy can be comparable with FAT. RT-PCR has the highest
sensitivity, therefore RT-PCR may be considered as the diagnostic test of the choice, in
FAT and dRIT negative samples. Histopathology cannot be considered as diagnostic test
because of its inconsistent findings of inclusions, where as IHC can replace
histopathology and can be used as one of the diagnostic test. Both Street and CVS rabies
infection was induced in mice to evaluate the pathomorphological changes and to
determine the expression of proinflammatory cytokines and their relation to clearance of
virus from brain tissue in mice. Histologically, in Street viral infection the lesions were
minimum and ware characterized by moderate microgliosis, PVC, satelittosis,
neuronophagia and presence of large number of Negribodies which were detected late in
infection, where as CVS infection was characterized by apoptosis of cells, degeneration
and necrosis of neurons, prominent inflammatory reaction, PVC with MNC and no Negri
bodies. The pro and anti inflammatory cytokines such as IL-1β, IL-6, INFγ, TNFα and
IL-10 showd increased expression. Similarly expression of rabies viral N gene occurs
with no difference in the expression between Street and CVS infected mice. A correlation
between the expression of cytokines and N gene amplification indicated that there is no
clearance of virus inspite of increased expression of cytokines in both Street and CVS
infections. The expression of pro and anti-inflammatory cytokines failed to clear the virus
from the brain as the expression of the rabies N gene did not reveal any decline and
increase in the expression of cytokines at the later stages of infection have no effect on
control of infection and death of the mice.
 
Date 2016-08-02T14:43:18Z
2016-08-02T14:43:18Z
2013-12-16
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/70591
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar