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Micropropagation of ginger (Zingiber officinale Rosc.)

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Title Micropropagation of ginger (Zingiber officinale Rosc.)
 
Creator Rajani C.Hiremath
 
Contributor S.S.Patil
 
Subject Horticulture
 
Description carried out during 2004-06 at the Tissue Culture Laboratory of Department of Horticulture,
College of Agriculture, University of Agricultural Sciences, Dharwad.
In the present investigation five sub experiments were carried out by following CRD
design and ‘t’ test in order to find out best surface sterilizer, explant, shooting media, rooting
media and hardening material.
Regarding the suitability of explants, shoot tip was the best for culture establishment
by producing more number of adventitious shoots in a shorter period of time i.e., early
emergence of primordial than the axillary bud.
The study on surface sterilization revealed that explants treated with 0.1 per cent
mercuric chloride for 12 minutes, showed the highest aseptic culture establishment. Among
the two different explants viz., shoot tips axillary buds treated, shoot tips gave maximum
survival percentage and healthy culture establishment.
Early response for sprouting and better culture establishment of shoot tip were
observed on Murashige and Skoog (MS medium). Among the cytokinins, BAP and kinetin at
different concentrations, 2.0 mg/l BAP. Produced more number of multiple shoots. Media
with the highest cytokinin concentration showed the maximum number of multiple shoots
and lowest length of shoots. On cytokinin free medium single shoot with maximum length
were produced.
Among the auxins used in the rooting experiment the maximum number of roots, with
less number of day taken for initiation were observed on 1 mg/l IBA supplemented medium.
NAA was found less effective than IBA. On auxin free medium maximum root length was
produced. Peat medium gave highest survival percentage at 15 and 30 days after transfer to
hardening media and better vigour of the plantlets were observed in peat media, followed by
vermiculture and sand media.
 
Date 2016-10-24T18:00:18Z
2016-10-24T18:00:18Z
2006
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/81624
 
Format application/pdf
 
Publisher UAS, Dharwad