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Development of thermophilic fungal consortium for enhancing biogas production from paddy straw

KrishiKosh

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Title Development of thermophilic fungal consortium for enhancing biogas production from paddy straw
 
Creator Nidhi
 
Contributor Phutela, Urmila Gupta
 
Subject enzymes, fungi, crop residues, rice, productivity, aromatic compounds, cellulose, biofuels, poultry equipment, extraction
 
Description The present study was aimed at developing and enriching thermophilic lignolytic fungal consortium for
efficient degradation of paddy straw. The effect of developed consortium on paddy straw digestibility and
biogas production was studied. A total of 80 thermophilic fungal cultures were isolated, purified and
screened qualitatively for ligno-cellulolytic potential on agar plates. Isolate T22 showed maximum
cellulase activity where as nineteen cultures showed positive results for lignin degradation. The twenty
isolates were further screened quantitatively for cellulolytic and lignolytic enzyme production. Eleven
consortia were formed from 5 isolates namely T5, T10, T14, T17 and T22. Paddy straw was pretreated
with individual cultures and developed consortium along with standard culture namely Humicola
fuscoatra MTCC 1409. H fuscoatra pretreated paddy straw showed 27.7% increase in biogas production
while T14, T17 and T22 pretreated paddy straw showed increase in biogas production by 37.0%, 41.6%
and 25.2%, respectively. The lignolytic enzyme production and change in chemical composition of paddy
straw pretreated with consortium (C10) made of isolates T14 (Aspergillus sp), T17 (Paecilomyces sp) and
T22 (Penicillium sp) was studied. Cellulose, hemicellulose, lignin and silica contents decreased
significantly with 57.01% increase in biogas production in 6 d pretreated paddy straw. Various parameters
like incubation temperature, pH, incubation time, metal ion (Cu+2) and nitrogen source (yeast extract)
were optimized for lignolytic enzyme production by Box Benhken model using Response Surface
Methodology (RSM) from consortium (C10). Maximum laccase (315.1 U/mg), lignin peroxidase (355.8
U/mg) and manganese peroxidae (238.7 U/mg) were produced from consortium (C10) when media was
supplemented with 0.3% Cu+2 and 0.1% yeast extract at pH 6.0, incubated at 50°C for 6 days. The
lignolytic enzymes were partially purified using ammonium sulphate precipitation, dialysis and ion
exchange chromatography using DEAE cellulose. There was 2.23 fold purification for laccase enzyme
with specific activity of 289.98 U/mg protein. The partially purified enzyme remained stable up to 96 h at
4°C. Hence the selected thermophilic fungal consortium (C10) was found to be suitable for increasing
paddy straw digestibility and biogas production.
 
Date 2016-04-11T14:40:44Z
2016-04-11T14:40:44Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65425
 
Language en
 
Format application/pdf
 
Publisher PAU