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STUDY OF TEMPERATURE STRESS TOLERANCE IN INDIAN MUSTARD (Brassica juncea L.)

KrishiKosh

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Title STUDY OF TEMPERATURE STRESS TOLERANCE IN INDIAN MUSTARD (Brassica juncea L.)
Ph D
 
Creator SUDHIR KUMAR
 
Contributor R.K. Sairam
 
Subject sowing, genotypes, planting, enzymes, developmental stages, fruits, tolerance, irrigation, brassica juncea, sowing date
 
Description T-8362
High temperature adversely affects photosynthesis, respiration, water relations and
membrane stability, and also modulate levels of hormones and primary and secondary
metabolites. Characterization of physiological traits imparting tolerance against high
temperature stress and identification of suitable screening indices and quantifiable traits
would facilitate the crop improvement process for temperature tolerance. Experiment
conducted with Fifteen Brassica juncea genotypes, subjected to temperature stress by
growing the crops at three dates of sowing showed decrease in relative water content (RWC),
total chlorophyll content and membrane stability index (MSI) at D2 and D3 sowings
compared to the D1. Yield attributes, such as number of primary branches, secondary
branches, no of pods per plant and seed yield also showed a similar trend as the level of
temperature stress increased at D2 and D3 sowings. It was concluded that parameters like
RWC, MSI and chlorophyll contents can be used as simple indices for screening and
identifying temperature tolerant genotypes. Increase in the activity of antioxidant enzymes
like superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR)
and catalase (CAT) in tolerant genotypes viz., Proagro, CS 52 and NDR 8801 under D3
sowing suggest that the antioxidant defense mechanism plays an important role in the heat
stress tolerance. Osmolytes also play major role in temperature stress tolerance in B. juncea
genotypes. Temperature stress due to late sowing (D2 and D3) resulted in increase in contents
of total sugar, proline, glycine-betaine and trehalose contents as compared to normal (D1)
sowing in tolerant genotypes. Gene Expression studied by RT-PCR showed temperature
stress induced increase in mRNA transcript of ∆-pyrroline-5-carboxylate synthetase (P5CS),
betaine aldehyde dehydrogenase (BADH) and trehalose-6 -phosphate synthase (T6PS), which
were again higher in Proagro, NDR 8801 and CS 52 compared to Pusa Agrani, EJ 15 and
Pusa Tarak. Results suggested that high temperature induced expression of P5CS, BADH and
T6PS in Proagro, NDR 8801 and CS 52 resulted in greater accumulation of osmolytes, which
could be one of the reasons for their tolerance to temperature stress
 
Date 2016-10-24T15:32:54Z
2016-10-24T15:32:54Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/81560
 
Format application/pdf
 
Publisher IARI, DIVISION OF PLANT PHYSIOLOGY