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Molecular analysis of male & female genotypes of Simarouba glauca DC using ISSR markers
KrishiKosh
View Archive Info| Field | Value | |
| Title |
Molecular analysis of male & female genotypes of Simarouba glauca DC using ISSR markers
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| Creator |
Yadav, Poonam
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| Contributor |
Dhillon, Santosh
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| Subject |
Simarouba glauca, Molecular polymorphism, Genetic diversity, Molecular markers, ISSR
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| Description |
Simarouba glauca is a tree born oilseed crop. It belongs to the family Simaroubaceae and is a native of El Salvador (Central America). The seeds of Simarouba are economically very important since they contain 55-60% edible oil. Simarouba is polygamodioecious with three types of plants namely pistillate (Female flowers), Staminate (Male flowers) and andromonoecious (male dominated bisexual flowers). As the waiting time from sowing to flowering is long, so the determination of sex of Simarouba seedling prior to flowering stage is necessary. Molecular markers are effectively and commonly being used in studying molecular polymorphism and genetic diversity in species. The present study was undertaken to assess molecular polymorphism among ten male, ten female and five bisexual genotypes of Simarouba glauca using ISSR markers. In the ISSR analysis of 25 genotypes using 40 ISSR primers, total 170 amplification products were obtained, out of which 34 are monomorphic and 136 are polymorphic. The polymorphism percentage ranged from 57.14% to 100%. The average polymorphism across all the 25 genotypes was found to be 87.6%. Overall size of PCR amplified products ranged between 150 bp-1700 bp. The similarity indices between different genotypes ranged from 0.43 to 0.93. These results indicate the presence of large genetic variation among the genotypes studied. Out of 40 ISSR primers that were surveyed to identify sex of Simarouba glauca, primer ISP815 was found to be male specific. This primer can be further converted into a SCAR marker which is more robust and will be of potential use in selecting plants of desired sex. The present study also demonstrates the utility and importance of ISSR markers in genetic diversity analysis and genotyping in Simarouba glauca. |
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| Date |
2016-09-12T09:08:25Z
2016-09-12T09:08:25Z 2013 |
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| Type |
Thesis
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/76372
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| Language |
en
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| Format |
application/pdf
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| Publisher |
CCSHAU
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