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MOLECULAR CHARACTERIZATION OF RICE GERMPLASM RESISTANT TO THE ASIAN RICE GALL MIDGE, Orseolia oryzae (WOOD-MASON)

KrishiKosh

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Title MOLECULAR CHARACTERIZATION OF RICE GERMPLASM RESISTANT TO THE ASIAN RICE GALL MIDGE, Orseolia oryzae (WOOD-MASON)
 
Creator SEKHAR DUTTA, SUVENDHU
 
Contributor BENTUR, J. S
 
Subject genes, rice, alleles, land resources, planting, genetics, land races, dna, fruits, diseases
 
Description Asian rice gall midge (Orseolia oryzae) is one of the major pests of rice causing
significant economic loss. Since chemical control of the pest is difficult and not
economical, cultivation of rice varieties possessing gall midge resistance is being practiced.
Extensive cultivation of varieties containing single resistance gene has resulted in frequent
breakdown of resistance due to emergence of virulent biotypes of the insect across many
locations in India. So far 11 gall midge resistance genes (Gm1, Gm2…...Gm11) and seven
biotypes of gall midge have been identified. Strategy of gene pyramiding has been
advocated to achieve durable gall midge resistance. The task of gall midge resistance gene
pyramiding through conventional breeding involving phenotype-based selection is very
difficult and sometimes impossible because of various reasons. Molecular markers linked
to the resistance genes can be helpful in accelerating gene pyramiding programmes, since
they facilitate selection of desirable genes without the need for phenotype based selection.
In the present study 97 test lines and 3 check lines of TN1were field evaluated against
the GMB3 at Jagtial, GMB4 at Ragolu, and GMB4M at Warangal, and GMB1 in
greenhouse test at DRR. But the pest pressure did not develop at Jagtial and Ragolu, so I
considered the data collected from Warangal and DRR. Further, gene specific markers
were chosen for genotypic analysis of these test lines. Considering the earlier reports gene
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specific flanking markers RM23956 and RM23914 were used for Gm1 gene, RM28574 and
RM235 for Gm11 gene detection. For gm3, Gm4 and Gm8 candidate gene based functional
markers Del3, LRR-Del and PRP were used, respectively. Considering both the phenotypic
reaction and genotypic results based on gene linked markers 17 entries showed nil damage
at the both locations and displayed known gene specific alleles while 31 entries showed
low damage at DRR and/ or Warangal. Among these six entries did not display any of the
known gene specific allele. Thus, these six entries could be caring a novel gene. Three of
these derived resistance from Orumundaken. Eighteen entries showed nil damage at DRR
but were susceptible at Warangal and 6 entries showed nil damage at Warangal but
susceptible at DRR; these entries need further evaluation. Rest 22 entries showed
susceptibility at both DRR and Warangal, these entries may not carry any of the R genes.
Flanking markers for Gm1 and functional marker PRP for Gm8 were stringent in detecting
the gene while Del3 marker appeared to be non-specific. Several of the test lines showed
two or more gene specific amplification- despite the earlier reports of these carrying one of
the specific gene.
Thus, either flanking SSR markers or gene specific functional markers can be
effectively used for detection of known genes or novel gene discovery. However, results
need to be carefully interpreted with sufficient phenotypic data and assured purity of the
test material.
 
Date 2016-06-13T12:17:02Z
2016-06-13T12:17:02Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67265
 
Language en
 
Relation D9314;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY