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COMPONENTS OF SPOT BLOTCH RESISTANCE IN RECOMBINANT INBRED LINES OF WHEAT (Triticum aestivum L.)

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Title COMPONENTS OF SPOT BLOTCH RESISTANCE IN RECOMBINANT INBRED LINES OF WHEAT (Triticum aestivum L.)
 
Creator Comfort Yusuf Sankem
 
Contributor Prof. Ramesh Chand
 
Subject Wheat, spot blotch, Histopathological and biochemical, molecular components
 
Description Ph. D.
Eighty seven recombinant inbred lines (F10) of “Yangmai 6 × Sonalika” cross and their parents were evaluated under field condition at the Agricultural Research Farm of Banaras Hindu University, Varanasi, India during two consecutive crop seasons 2013/14 and 2014/15. The RILs were grouped into two, based on phenotypic features i.e. Sonalika and Yangmai 6 type to avoid the confounding of the background effect on resistance or susceptibility. The eighty seven recombinant inbred lines (RILs) along with their parents Sonalika and Yangmai 6 genotypes were used to identify the resistance components associated with resistance and susceptibility to spot blotch (B. sorokiniana). To achieve this objective, resistance components viz., area under the disease progress curve (AUDPC), flag leaf lesion size, spore number cm-2 lesion in flag leaf, spore load 50-1 seed, lesion number on flag leaf, latent period, infection efficiency, thousand kernel weight, lesion mimic, leaf tip necrosis, and days to heading were evaluated . Significant differences were obtained among RILs and between years for all resistance components.
The correlation coefficient (r) of the components revealed significant correlation between some of the components, for instance the correlation between Disease severity with AUDPC, lesion size, lesion mimic, lesion number, and spore number showed a positively significant correlation. While a negative significant correlation was obtained with thousand kernel weight (TKW), days to heading (DH) and latent period (LP).
The molecular characterization revealed presence of lm, lm1, lm2, Lr34, Lr46, vrn1 and ppd1 genes in most of the lines. It has been noted in this study that 3 genes altogether (lm, lm1 and lm2) reduced the level of expression of lesion mimic, hence low AUDPC was observed. The percentages of RILs that acquired the Lr34 and Lr46 genes were 50.6% and 49.4% respectively. All the parent and RILs possess the vrn1 and ppd1 genes. These genes could be consider important to wheat genotypes in South Asia to develop resistant variety. In conclusion, the resistant components such as Area under disease progress curve, lesion size, days to heading, lesion mimic, latent period, infection efficiency in combination with the histo- pathological and biochemical could be incorporated in breeding for resistance against spot blotch in South Asia. The molecular evidences, such as the presence of lm, lm1, lm2, Lr34 Lr46, vrn1 and ppd1 from this study provided useful information that can be used for screening for better adaptability, maturity and resistance against spot blotch.
 
Date 2016-09-21T19:47:03Z
2016-09-21T19:47:03Z
2016
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/78238
 
Language en
 
Relation P0042;
 
Format application/pdf
 
Publisher Institute of Agricultural Sciences, Banaras Hindu University