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G and P typing and sequence analysis of group A rotavirus in calves and lambs in Kashmir

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Title G and P typing and sequence analysis of group A rotavirus in calves and lambs in Kashmir
 
Creator Hassan, Mir Nadeem
 
Contributor Wani, Shakil Ahmad
 
Subject Bovine, Ovine group A rotavirus, G genotype, P genotype, PCR, RNA-Page
 
Description The present study was carried out on the distribution, relative frequency of
G and P genotypes of bovine, ovine and human group A rotaviruses and to
determine variation if any within these G & P types. A total of 580 samples from
290 diarrhoeic calves, 145 diarrhoeic lambs and 145 diarrhoeic children were
collected and screened for the presence of rotavirus. Out of 290, 145 and 145
diarrhoeic stool samples from calves, lambs and children, 37, 12 and 39 were
found positive for Group A rotavirus, respectively by latex agglutination test.
Electropherotyping by ribonucleic acid-polyacrylamide gel electrophoresis
revealed the typical group A rotavirus 4-2-3-2 migration pattern in 31, 7 and 34
diarrhoeic stool samples from calves, lambs and children, respectively. Out of 37,
12 and 39 latex aggultination test positive samples from calves, lambs and
children, 34, 8 and 37, respectively were further processed for G and P
genotyping. The G10 genotype was the most predominant G genotype in bovines
accounting for 35.3% of the samples; G6, G3 and G8 genotypes were found in
29.4, 17.6 and 5.9% of the bovines samples, respectively. One sample (2.9%)
revealed a mixed infection with G6 and G8 genotypes, whereas six (8.9%) of
bovine samples could not be characterised with 10 G genotype-specific primers
used. Determination of P genotype specificities in bovines revealed P[11] genotype in 67.6% of samples. P[1] and P[5] genotypes were found in 5.9 and
17.6% of bovine samples, respectively. G10P[11] (26.5%) was the most frequent
G and P genotype combination in bovines, while G6P[11], G3P[11], G10P[5],
G3P[5], G6P[1] and G6P[5] were found in 20.6, 11.8, 8.8, 5.9, 5.9 and 2.9% of
the the bovine rotavirus samples, respectively. One (2.9%) sample showed a
mixed infection with G6+G8P[11] specificities. The present study in ovines
revealed G6 to be the most predominant (62.5%) G genotype followed by G10
(37.5%). Among P genotype in ovines, all (100%) revealed P[11] genotype. The
most predominant G and P combination in ovines was G6P[11] (62.5%) followed
by G10P[11] (37.5%). The investigation in human revealed G1 to be the most
predominant G genotype with a frequency of 46.9%. G2 and G9 were found in
13.5 and 8.1% of the human rotavirus positive samples, respectively. Among P
genotypes in humans, P[4] was the predominant one with a frequency of 45.9%
followed by P[6] (29.7%). G genotype could not be ascertained in five (13.5%)
human samples and P genotype in other 9 (24.3%) samples. Among humans, most
predominant G and P combination was G1P[4] (35%), while G1P[6], G2P[4],
G2P[6], G9P[4], G9P[6], G1P[UT], G2P[UT] and P[6]G(UT) were found in 10.8,
5.4, 2.7, 5.4, 2.7 ,18.9, 5.4 and 13.5% of the rotavirus samples, respectively.
Phylogenetic analysis of JKBRVP7-1/Bovine/2013/India, JKBRVP4/2013/ India
and JKBR/India/ 2013/P[11] detected in this study indicated that these genotypes
were genetically distant from other so far reported rotavirus genotypes suggesting
possible emergence of new genotypes.
 
Date 2016-08-19T14:40:51Z
2016-08-19T14:40:51Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/73049
 
Language en
 
Format application/pdf
 
Publisher SKUAST