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Multilocus Sequence Typing Of Listeria Monocytogenes From Animal And Human Clinical Cases

KrishiKosh

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Title Multilocus Sequence Typing Of Listeria Monocytogenes From Animal And Human Clinical Cases
 
Creator Bobade, S. S.
 
Contributor Kalorey, D. R.
 
Subject Veterinary Biotechnology
Multilocus Sequence Typing Of Listeria Monocytogenes From Animal And Human Clinical Cases
 
Description In the present investigation, prevalence and the virulence gens profiling of
Listeria monocytogenes isolates recovered from infertility cases of women was studied by
employing the polymerase chain reaction. Total of 113 endocervical and vaginal swabs were investigated for the isolation
of L.monocytogenes . The isolation was attempted by two step enrichment procedure in
UVM-1 and UVM 2 enrichment broths followed by plating on DRIA.. The L.
monocytogenes isolates were tested for detection of virulence-associated gens namely
plc A, act A, hly A and iap by multiplex PCR and prf A by singleplex PCR . Prevalence of
L. monocytogenes were found to be 9.73% in infertility cases. Most of the isolates were
positive for act A, hly A and prf A .
L. monocytogenes strain isolated from human and animal(clinical cases), meat,milk
and vegetables sources were studied for MLST analysis. Singleplex PCR was
standerised and employed for study of five housekeeping genes viz. abc Z,bgl,dape,dat
and ldh of L.monocytogenes..The amplified gene fragments were sequenced .The
nucleotide sequence obtained were compared with existing nucleotide sequence at NCBI,
which reveals maximum identity up to ( 88%-99%).
Neighbor-joining tree based on nucleotide difference in five housekeeping genes
most of L.monocytogenes isolates were clustered in one major clad .
The nucleotide sequence data was submitted at MLST database (www.paster
.fr/mlst) . The MLST analysis of five housekeeping genes indicated discrimination index
range between 0.2 to 0.533 .Housekeeping gene ldh has better discrimination power
(0.533) ,while dape gene showed poor discrimination power.
 
Date 2017-01-03T16:58:45Z
2017-01-03T16:58:45Z
2012-09-16
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94276
 
Language en
 
Format application/pdf
 
Publisher MAFSU