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Utilization of xylose for bioethanol production

KrishiKosh

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Title Utilization of xylose for bioethanol production
 
Creator Singla, Ankit
 
Contributor Dhamija, S.S.
 
Subject Bacteria, Productivity, Carbon, Genes, Nitrogen, Biological development, Dna, acidity, Inorganic acid salts, Byproducts
 
Description The present investigation was undertaken to isolate xylose fermenting yeasts
and examining their ability to ferment xylose in the presence of glucose. Seventeen
yeast isolates were retrieved from different ecological niches. Grapes were found to be
the most suitable source offering 5 isolates. Based on absorbance values various
constituents of xylose- containing inoculum medium were optimized. The optimum
xylose concentrations of inoculum and production media were found to be 1 and 10 %,
respectively. All the isolates were subsequently screened for ethanol production on
xylose medium. While seven isolates produced no alcohol at all on xylose, ten isolates
produced 1-2 % (v/v) ethanol on xylose and 3-5 % (v/v) ethanol on dextrose. All the six
yeast isolates which produced 5 % (v/v) ethanol on dextrose were screened for ethanol
production on a medium containing both dextrose and xylose (4:3 ratio). Isolate 11
produced the maximum (4.5 %) ethanol from mixed carbon sources leaving behind only
0.5 % residual sugars, which seemed to represent xylose rather than dextrose. It was
further examined for ethanol production on media containing either dextrose or both
dextrose and xylose at 12 and 15 % sugar levels. In the latter, the two sugars were
always in a ratio of 4 parts dextrose and 3 parts xylose. In case of the fermentation
medium containing dextrose, a maximum of 5.5 % (v/v) ethanol was achieved at an
initial sugar concentration of 12 % at 72 h. Similarly, on the medium containing both
dextrose and xylose, a maximum of 4.7 % (v/v) ethanol was obtained at an initial sugar
concentration of 12 % at 72 h, Based on the morphological and biochemical properties
of isolate 11, the results were found to conform well with the characteristic properties of
Candida.
 
Date 2016-11-11T13:28:42Z
2016-11-11T13:28:42Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85081
 
Language en
 
Format application/pdf
 
Publisher CCSHAU