Evaluation of Tumor Induced Angiogenesis in Rat and Canine Mammary Gland Tumors.
KrishiKosh
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Title |
Evaluation of Tumor Induced Angiogenesis in Rat and Canine Mammary Gland Tumors.
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Creator |
Mohankumar Shettar
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Contributor |
Suguna Rao
Ananda Kumar, A. Satyanarayana, M.L. Byregowda, S.M. Suryanarayana, V.V.S. Ramachandra, S.G. Shridhar, N.B. |
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Subject |
DMBA, Angiogenesis, RT PCR, Immunohistochemistry
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Description |
Ph.D. Thesis
Angiogenesis is a vital process essential for tumour growth, invasion and metastasis and is regulated by many factors of which, VEGF and MMP2 are the most important ones. The present study was carried out to evaluate tumour induced angiogenesis in spontaneous canine and induced rat mammary tumours, based on immunohistochemical and real time PCR expression of VEGF and MMP2. In rats, tumours were induced by oral administration of DMBA. The most common histological type of mammary tumour encountered in canine and rats was papillary adenocarcinoma. The expression of VEGF and MMP2 was observed by both immunohistochemical and PCR methods in all the tumour types of both canine and rat mammary tumours. The expression was higher in malignant tumours compared to benign and non-neoplastic growths. Among malignant tumours the expression of VEGF and MMP2 varied between the tumour types. In canine mammary tumours the expression of VEGF was highest in papillary adenocarcinoma complex type and MMP2 in papillary adenocarcinoma and adenosquamous carcinoma. In rat mammary tumours the VEGF and MMP2 expressions were highest in papillary adenocarcinoma. The results suggested that a higher angiogenic process occurred in malignant types, which correlated, with increased microvasculatures in histological preparations. Comparison of tumour induced angiogenesis between canine and rat mammary tumours indicated that the process of angiogenesis did not vary between them and VEGF and MMP2 occurred as independent factors. |
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Date |
2016-08-02T13:55:21Z
2016-08-02T13:55:21Z 2012-04-17 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/70556
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Language |
en
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Format |
application/pdf
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Publisher |
Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
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