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Characterization and development of molecular marker for the detection of Fusarium oxysporum f. sp ciceries causing chickpea wilt

KrishiKosh

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Title Characterization and development of molecular marker for the detection of Fusarium oxysporum f. sp ciceries causing chickpea wilt
Ph D
 
Creator DURAI, M
 
Contributor S. C. DUBEY
 
Subject fungi, fusarium oxysporum, dna, diseases, chickpeas, fruits, rapd, irrigation, solutes, water
 
Description T-8384
Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris is a serious and wide
spread disease of chickpea (Cicer arietinum) in all chickpea growing countries.
Virulence analysis of 36 isolates of the pathogen collected from 12 states of india, on a
new set of 10 differential cultivars of chickpea, namely, C104, JG 74, CPS 1, PUSA
212, WR 315, KWR 108, GPF 2, DCP 92-3, Chaffa and JG 62, grouped them into
eight races. Except the isolates from Delhi, Haryana, Bihar, Uttar Pradesh and Madhya
Pradesh, the races/virulence groups were corresponding to the area of the origin of the
isolates. Genetic diversity of 14 representative isolates of pathogen determined through
internal transcribed spacer (ITS) region of rDNA - Restriction Fragment Length
Polymorphism(ITS-RFLP) and amplified products were digested with 7 restriction
enzymes (Tru I, AluΙ, Rsa I, Kpn I, Eco RI, Sal I and Bam H I). Some of the isolates
gave area specific ITS-RFLP patterns, whereas others from the same area showed
distinct pattern and matched with the pattern of the isolates of the other areas. Clearly
indicating the presence of highly variable population in chickpea growing areas of India.
The ITS-RFLP pattern was partially related with the geographical origin of the isolates.
The phylogenetic analysis based on ITS sequences of the isolates also grouped the
grouped them into eight categories which were partially corresponding to the virulence
groups or races of the pathogen. Genetic diversity of the population of F. oxysporum
f.sp ciceris was also determined through random amplified polymorphic DNA (RAPD)
markers. Unweighted paired group method with arithmetic average (UPGMA) cluster
analysis of RAPD profiles grouped the isolates into eight categories showing high
magnitude of genetic diversity. Each group had the isolates from different states of
India. The molecular groups were not corresponding to the geographical origin of the
isolates. The RAPD primer OPA 07 and OPA 11 produced specific fragments of 1.3 kb
and 1.4 kb, repectively in all the isolates of the pathogen. Primers were designed with
sequence information of these two fragments using primer 3 software. Two sets of
Sequence characterized amplified region (SCAR) markers (CW-FOC 1 and CW-FOC
2) developed from the sequences of these fragments were found to be specific to F.
oxysporum f. sp ciceris and produced an amplicon of 1.3 kb and 1.4 kb respectively.
These set of markers were validated against the isolates of the pathogen collected from
different locations of India, representing various races of the pathogen. First time SCAR
markers have been developed to detect for Indian population of Fusarium oxysporum f.
sp. ciceris through the present study.
 
Date 2016-10-31T13:31:31Z
2016-10-31T13:31:31Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/82982
 
Format application/pdf
 
Publisher IARI, DIVISION OF PLANT PATHOLOGY