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Genetic Investigations in Gowli Buffaloes of Karnataka

KrishiKosh

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Title Genetic Investigations in Gowli Buffaloes of Karnataka
 
Creator Jayashankar M.R.
 
Contributor Govindaiah M.G.
 
Subject livestock, chromosomes, breeds (animals), dna, genetics, rapd, polymorphism, pcr, genomes, sampling
 
Description Morphological traits and body measurements were recorded in 129 Gowli
buffaloes. The average height at withers, body length, heart girth and body girth were
124.9 ± 1.20 and 124.0 ± 1.73 cm, 136.1 ± 1.51cm and 129.3 ± 4.51 cm, 189.4 ± 2.11 cm
and 171.0 ± 7.00 cm and 206.9 ± 1.86 cm and 177.0 ± 4.65 cm for adult females above
four years and breeding bulls, respectively.
Chromosome analysis of Gowli buffaloes revealed a modal chromosome number
of 2n = 50. The karyotype comprised of 5 pairs of submetacentric and 19 pairs of
acrocentric autosomes and a pair of acrocentric sex chromosomes. The X chromosome
was the largest acrocentric. The Y chromosome was identified as the smallest
acrocentric. The mean relative length (%) of the autosomes varied from 1.70 ± 0.09 to
7.90 ± 0.17. The mean relative length of X chromosome was approximately equal to that
of 4th pair of autosomes. The centromeric index value was highest for fifth pair and
lowest for first pair of autosomes. The banding patterns were in close agreement with the
G-band features reported earlier in river type buffaloes.
RAPD analysis was performed on 50 animals each of Gowli and South Kanara
buffaloes using 10 random primers by PCR technique. Eight primers primers (ILO 868,
ILO 876, OPG 05, OPG11, OPG 13, OPAV 15, OPAX 19 and BG 28) were found to
produce moderate to high polymorphism in South Kanara buffaloes than in Gowli
buffaloes. All the fragments observed in Gowli buffaloes were detected in South Kanara
buffaloes. An additional fragment of 918 bp was observed with primer OPG 11 only in
Gowli buffaloes. Primers ILO 868, ILO 876 and BG 28 were found useful for
differentiating Gowli and South Kanara buffaloes, with primer ILO 876 being the primer
of choice.
 
Date 2016-06-17T12:47:15Z
2016-06-17T12:47:15Z
2005-08-10
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67530
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar