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Molecular Genetic Studies on Butyrophilin Gene in Malnad Gidda Cattle and its Association with Milk Quality Traits

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Title Molecular Genetic Studies on Butyrophilin Gene in Malnad Gidda Cattle and its Association with Milk Quality Traits
 
Creator Vishwanath, B.
 
Contributor Nagaraja, C.S.
Jayashankar, M.R.
Narasimha Murathy
Shri Krishna Isloor
 
Subject Molecular Genetic Studies on Butyrophilin Gene in Malnad Gidda Cattle and its Association with Milk Quality Traits
 
Description PG. Thesis
The present work was aimed at studying the Butyrophilin gene polymorphism and
its association with milk quality traits in Malnad Gidda cattle. Blood and milk samples
were collected from 50 Malnad Gidda cattle from Uttar Kannada district and
Chickmagaloor districts of Karnataka. Milk samples were collected at fortnight intervals,
for six times from the same animals. Genomic DNA was isolated by Miller’s High Salt
method and Fat, SNF and Total Ash were analyzed by Gerber method, Formula method
and Gravimetric method respectively.
The genetic polymorphism of Butyrophilin gene was investigated by PCR-RFLP
technique. An 893bp fragment of exon 8 of Butyrophilin gene was amplified. The
amplified products were digested with restriction enzyme HaeIII. The restricted products
were subjected to three per cent agarose gel electrophoresis and revealed two alleles A
and B and genotypes AA and AB. The genotype BB was absent in the population studied.
The highest gene and genotype frequencies were observed for A allele (0.88) and AA
genotype (0.76) and lowest gene and genotype frequencies were observed for B allele
(0.12) and AB genotype (0.24) respectively. The population studied was in Hardy-
Weinberg equilibrium. Sequence analysis indicated 100 % homology to B.taurus and 99
per cent to B.indicus. There was a significant association between milk quality traits like
Fat and SNF with genotypes AA and AB where A allele was gene was favorable,
whereas total ash was not associated with any genotype. The present study revealed the
polymorphism of BTN1A1 gene and its association with milk quality traits.
 
Date 2016-11-14T15:49:07Z
2016-11-14T15:49:07Z
2014-05-15
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85452
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar