Study on Beta Casein Polymorphism in Malnad Gidda and Crossbred Cattle
KrishiKosh
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Title |
Study on Beta Casein Polymorphism in Malnad Gidda and Crossbred Cattle
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Creator |
Navyashree, T.C.
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Contributor |
Nagaraja, C.S.
Jayashankar, M.R. Satyanarayan, K. Shettar, V.B. |
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Subject |
Study on Beta Casein Polymorphism in Malnad Gidda and Crossbred Cattle
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Description |
PG. Thesis
The exon 7 region of beta casein gene of Malnad Gidda and crossbred cattle was studied with an objective of analyzing its polymorphism. PCR–RFLP analysis of the amplified region revealed monomorphic pattern in Malnad Gidda and polymorphic pattern in crossbred cattle. Only A2A2 genotype was found in Malnad Gidda cattle. A2 allele was predominant in crossbred cattle with an allelic frequency of 0.79 while A1 allelic frequency was 0.21. The frequencies of A1A1, A1A2 and A2A2 genotypes were 0.13, 0.18 and 0.69 respectively, in crossbred cattle. The Malnad Gidda population was in Hardy Weinberg equilibrium indicating absence of selection pressure while crossbred population was not in equilibrium. The sequence analysis confirmed the difference between A1 and A2 allele due to SNP resulting in substitution of ‘C’ to ‘A’ causing replacement of proline by histidine. The present study confirmed the theory that A2 allele of beta casein is predominant in Bos indicus and crossbred cattle possess both A1A2 heterozygotes and A2A2 homozygotes. |
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Date |
2016-11-14T15:53:01Z
2016-11-14T15:53:01Z 2014-08-12 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/85454
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Language |
en
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Format |
application/pdf
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Publisher |
Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
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