ICAR Research Data Repository for Knowledge Management
Genetic Investigations in Gowli Buffaloes of Karnataka
KrishiKosh
View Archive Info| Field | Value | |
| Title |
Genetic Investigations in Gowli Buffaloes of Karnataka
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| Creator |
Jayashankar M.R.
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| Contributor |
Govindaiah M.G.
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| Subject |
aloe, fruits, extraction, biological phenomena, guavas, sweeteners, sugar, acidity, beverages, irrigation
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| Description |
Morphological traits and body measurements were recorded in 129 Gowli buffaloes. The average height at withers, body length, heart girth and body girth were 124.9 ± 1.20 and 124.0 ± 1.73 cm, 136.1 ± 1.51cm and 129.3 ± 4.51 cm, 189.4 ± 2.11 cm and 171.0 ± 7.00 cm and 206.9 ± 1.86 cm and 177.0 ± 4.65 cm for adult females above four years and breeding bulls, respectively. Chromosome analysis of Gowli buffaloes revealed a modal chromosome number of 2n = 50. The karyotype comprised of 5 pairs of submetacentric and 19 pairs of acrocentric autosomes and a pair of acrocentric sex chromosomes. The X chromosome was the largest acrocentric. The Y chromosome was identified as the smallest acrocentric. The mean relative length (%) of the autosomes varied from 1.70 ± 0.09 to 7.90 ± 0.17. The mean relative length of X chromosome was approximately equal to that of 4th pair of autosomes. The centromeric index value was highest for fifth pair and lowest for first pair of autosomes. The banding patterns were in close agreement with the G-band features reported earlier in river type buffaloes. RAPD analysis was performed on 50 animals each of Gowli and South Kanara buffaloes using 10 random primers by PCR technique. Eight primers primers (ILO 868, ILO 876, OPG 05, OPG11, OPG 13, OPAV 15, OPAX 19 and BG 28) were found to produce moderate to high polymorphism in South Kanara buffaloes than in Gowli buffaloes. All the fragments observed in Gowli buffaloes were detected in South Kanara buffaloes. An additional fragment of 918 bp was observed with primer OPG 11 only in Gowli buffaloes. Primers ILO 868, ILO 876 and BG 28 were found useful for differentiating Gowli and South Kanara buffaloes, with primer ILO 876 being the primer of choice. |
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| Date |
2016-06-17T11:47:38Z
2016-06-17T11:47:38Z 2005-08-10 |
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| Type |
Thesis
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/67523
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| Language |
en
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| Format |
application/pdf
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| Publisher |
Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
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