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Dye decolorization and industrial effluent lignin degradation by free and immobilized fungal species

KrishiKosh

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Title Dye decolorization and industrial effluent lignin degradation by free and immobilized fungal species
 
Creator Verma, Megha
 
Contributor Rai, J.P.N.
 
Subject dyes, decolorization, industrial effluents, lignin, degradation, immobilization, fungus, species diversity, pulp and paper industry, chromatography, biomass
 
Description Thesis-PhD
Three fungal isolates MV01, MV02 and MV04 were taken for industrial effluent lignin degradation and dye decolorization process and these isolates were selected from rotten apple, wood chip and Eucalyptus tree trunk. The fungal isolates were identified on the basis of ITS region and 18S r RNA gene sequencing respectively, viz Rhizopus oryzae (MV01), Aspergillus niger (MV02) and Mucor heimilis (MV04). Two types of fungal biomass were constructed one free and one immobilized biomass. MV02 fungal isolates showed maximum deligninification and dye decolorization potential as compare to MV01 and MV04 fungal isolates. Among both immobilization matrix (sodium alginate and orange peeling) orange peeling showed maximum delignification and dye decolorization potential. Maximum reduction in lignin and color were recorded in concentration 20 mg l-1 and 5 mg l-1. Optimum nutrient condition were observed as dextrose (carbon source), sodium nitrate (nitrogen source). 30O C temperature was the best for dye decolorization and dye decolorization for all fungal strains. pH 6.0 and agitation speed 140 rpm for delignification and pH 5.0 and agitation speed 130 rpm was recorded for dye decolorization study. In present study pulp and paper mill effluent color reduction and biomass increase during dye decolorization were recorded. All the results showed that maximum reduction was observed in free and immobilized biomass of MV02 fungal strains. Immobilization gives better operational stability and higher efficiency. Scanning electron microscopy (SEM) analysis of free and immobilized MV02 fungal strain reveled that immobilized hyphae showed coiling and increase in the size of conidia. In immobilized strain conidia showed clump structure. Three consortiums were formed (MV01+MV02, MV01+MV04, MV02+MV04) and result obtained that consortium showed more delignification and dye decolorization compare to free biomass but less for immobilized biomass. The sequential treatment with co-immobilized fungal biomass showed remarkable improvement in degradation performance and resulted in change in pH and lignin. Total ion chromatography obtained from GC/MS analysis affirms degradation of majority of the compound present in Pulp and Paper mill effluent
 
Date 2016-07-26T15:45:23Z
2016-07-26T15:45:23Z
2015-07
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69962
 
Language en
 
Format application/pdf
 
Publisher G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)