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BIOPROSPECTING OF Xanthomonas axonopodis pv. punicae STRAINS FOR PRODUCTION OF CELLULASES AND XYLANASE INOLVED IN SACCHARIFICATION

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Title BIOPROSPECTING OF Xanthomonas axonopodis pv. punicae STRAINS FOR PRODUCTION OF CELLULASES AND XYLANASE INOLVED IN SACCHARIFICATION
M Sc
 
Creator DOLAMANI AMAT
 
Contributor Anju Arora
 
Subject enzymes, productivity, bacteria, cellulose, polysaccharides, fungi, sugar, biomass, planting, extraction
 
Description T-8664
Xanthomonas axonopodis p.v punicae strain (a potent plant pathogen which causes
blight disease in pomegranate) was screened for cellulolytic and xylanolytic enzyme
production. This strain produced CMCase (endo-β-1, 4-glucanase), FPase, xylanase and
cellobiase specific activities (IU/mg of protein) i.e. 0.122, 0.168, 0.744 and 0.82
respectively. Enzyme production was optimized with respect to major nutrient sources like
Carbon and Nitrogen. Carboxy Methyl Cellulose (CMC) was found to be better C source for
CMCase and Xylanase production, starch was found to be good carbon source for production
of cellobiase production and α-cellulose was found to stimulate FPase activities
production. Soyabean meal and yeast extract were better nitrogen sources for both
cellulolytc and xylanolytic enyme production except cellobiase (β-D-glucosidase) which
was higher with peptone as nitrogen source. Surfactants had no significant effect on level of
extracellular enzymes production. Temperature 28°C and pH 6-8 was found to be optimum
for production of cellulolytic and xylanolytic enzymes production by this strain. Enzymes
produced under optimized condition resulted in around 4 and 5 fold increase in FP activity
(exo-β-1,4-glucanase) and CMCase activities respectively while 1.72 fold increase was
observed in case of xylanase and cellobiase activities. Physio-chemical characterization of
enzymes produced under optimized condition showed that all the enzymes had activity over
broad range of pH 4-8 with pH optimum of 8. FP activity and xylanase showed two pH
optima showing more isoforms of enzymes involved. Cellulolytic enzymes showed
temperature optimum around 55°C while xylanase activity was highest at 45°C and declined
as temperature increased . Xylanase activitiy was more stable than cellulolytic activities after
heat treatment at 75°C for 1 hr. Thus the enzymes were versaltile with respect to their pH
and temperature optima and were thermostable .These enzymes can be overproduced and can
find application in different industries including food, pulp and paper and biorefineries for
conversion of lignocellulosic biomass.
 
Date 2016-09-12T18:26:10Z
2016-09-12T18:26:10Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/76474
 
Format application/pdf
 
Publisher IARI, DIVISION OF MICROBIOLOGY