BIOPROSPECTING OF Xanthomonas axonopodis pv. punicae STRAINS FOR PRODUCTION OF CELLULASES AND XYLANASE INOLVED IN SACCHARIFICATION
KrishiKosh
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Title |
BIOPROSPECTING OF Xanthomonas axonopodis pv. punicae STRAINS FOR PRODUCTION OF CELLULASES AND XYLANASE INOLVED IN SACCHARIFICATION
M Sc |
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Creator |
DOLAMANI AMAT
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Contributor |
Anju Arora
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Subject |
enzymes, productivity, bacteria, cellulose, polysaccharides, fungi, sugar, biomass, planting, extraction
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Description |
T-8664
Xanthomonas axonopodis p.v punicae strain (a potent plant pathogen which causes blight disease in pomegranate) was screened for cellulolytic and xylanolytic enzyme production. This strain produced CMCase (endo-β-1, 4-glucanase), FPase, xylanase and cellobiase specific activities (IU/mg of protein) i.e. 0.122, 0.168, 0.744 and 0.82 respectively. Enzyme production was optimized with respect to major nutrient sources like Carbon and Nitrogen. Carboxy Methyl Cellulose (CMC) was found to be better C source for CMCase and Xylanase production, starch was found to be good carbon source for production of cellobiase production and α-cellulose was found to stimulate FPase activities production. Soyabean meal and yeast extract were better nitrogen sources for both cellulolytc and xylanolytic enyme production except cellobiase (β-D-glucosidase) which was higher with peptone as nitrogen source. Surfactants had no significant effect on level of extracellular enzymes production. Temperature 28°C and pH 6-8 was found to be optimum for production of cellulolytic and xylanolytic enzymes production by this strain. Enzymes produced under optimized condition resulted in around 4 and 5 fold increase in FP activity (exo-β-1,4-glucanase) and CMCase activities respectively while 1.72 fold increase was observed in case of xylanase and cellobiase activities. Physio-chemical characterization of enzymes produced under optimized condition showed that all the enzymes had activity over broad range of pH 4-8 with pH optimum of 8. FP activity and xylanase showed two pH optima showing more isoforms of enzymes involved. Cellulolytic enzymes showed temperature optimum around 55°C while xylanase activity was highest at 45°C and declined as temperature increased . Xylanase activitiy was more stable than cellulolytic activities after heat treatment at 75°C for 1 hr. Thus the enzymes were versaltile with respect to their pH and temperature optima and were thermostable .These enzymes can be overproduced and can find application in different industries including food, pulp and paper and biorefineries for conversion of lignocellulosic biomass. |
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Date |
2016-09-12T18:26:10Z
2016-09-12T18:26:10Z 2012 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/76474
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Format |
application/pdf
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Publisher |
IARI, DIVISION OF MICROBIOLOGY
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