Record Details

Structure, Morphology, Biochemical Composition of Scelerotia and Molecular Variability in Sclerotium rolfsii Sacc. Form Groundnut

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Structure, Morphology, Biochemical Composition of Scelerotia and Molecular Variability in Sclerotium rolfsii Sacc. Form Groundnut
 
Creator Venkatesh Tiramsetti
 
Contributor S.S. Adiver
 
Subject Plant pathology
 
Description Groundnut (Arachis hypogea L.) is an annual legume and important oilseed crop
grown in India. Stem rot disease caused by Sclerotium rolfsii Sacc. is posing serous threat to
crop production. There was a gradual change in structure and morphological characters of
sclerotia upon maturity. Size of immature sclerotia was 1911.76 μm (maximum), in partial
mature sclerotia 1733.34 μm and in mature sclerotia 1562.72 μm. The levels of biochemical
constituents in sclerotia were changed with respect to different stages of sclerotia. The
reducing and non reducing sugar contents were increased, phenols and tannins contents were
unchanged while protein and free amino acids were reduced with increasing age of sclerotia.
Bleaching powder showed maximum inhibition on germination of sclerotia (97.70%)
followed by gypsum (17.04%) and NSKE (9.64 %). Parthenium leaf extract (10%) was most
effectively inhibited the sclerotial germination (31.67%) and delayed the period of
germination up to 130.22 h. Pendimethalin (200 ppm) showed highest per cent inhibition of
sclerotial germination (17.40%) and also delayed germination longer period (98.67 h). While
ethylene enhanced the germination per cent and reduced the period of germination
irrespective of sclerotial stages. Maximum inhibition of sclerotial germination was noticed
with Trichoderma viride (63.33%) compared to T. harzianum (55.65%). Similarly
Pseudomonas fluorescens inhibited maximum germination (90%) than Bacillus subtilis
(81.11%) different ages of sclerotia.
Ten isolates of S. rolfsii showed variability in morphological characters. The
maximum colony diameter (85.47 mm) was attained by Coimbatore isolate and higher dry
mycelia weight was recorded in Akola isolate (829.32 mg). The Coimbatore isolate produced
maximum length of infection (10.70 cm) on TMV-2 variety and least by Hospet isolate (7.30
cm). Molecular variability studies by RAPD revealed that highest similarity observed
between Belgaum-Coimbatore isolates and least similarity in Prakasam-Akola isolates.
 
Date 2016-11-22T12:53:36Z
2016-11-22T12:53:36Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/86929
 
Format application/pdf
 
Publisher UAS, Dharwad