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STUDIES ON BIOLOGICAL SEED COATING IN PIGEON PEA (Cajanus cajan L.)

KrishiKosh

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Title STUDIES ON BIOLOGICAL SEED COATING IN PIGEON PEA (Cajanus cajan L.)
 
Creator JAGADEESH, VARADA
 
Contributor SUJATHA, P
 
Subject “STUDIES, BIOLOGICAL, SEED COATING, PIGEON PEA
 
Description The present investigation entitled studies on Biological seed coating in Pigeon pea was carried out during Kharif, 2015 and Rabi, 2015-16 in the department of Seed Science and Technology, Rajendranagar, Hyderabad and the department of Agriculture Microbiology and Bio-energy, College of Agriculture, PJTSAU, Rajendranagar, Hyderabad to study the viability and shelf life of biological agents on the seed quality and storability. Pigeon pea (LRG-41) seed was coated with four biological organisms like Trichoderma viride, Pseudomonas fluorescence, Rhizobium and PSB by using two adjuvants like bio friendly polymer and sugar syrup and the treated seed was kept for six months storage. The viability and shelf life of biological agents were tested through Colony Forming Units (CFU) count throughout the storage period of biologically coated seed of pigeon pea.
Trichoderma viride and Rhizobium showed lower colony counts with biofriendly polymer (19.4 x 105 cfu g-1 and 16.3 x 105 cfu g-1, respectively) and Pseudomonas and PSB showed higher colony counts (12.6 x 105 cfu g-1 and 13.8 x 105 cfu g-1, respectively) when compared to their counts in the bio formulations. In combinational treatments of Trichoderma viride with bio fertilizers, Trichoderma viride showed lower count (18.4 x 105 cfu g-1) but Rhizobium showed higher count (16.8 x 105 cfu g-1) in one combination. Whereas, in another combination, Trichoderma viride showed still lower count (13.0 x 105 cfu g-1) and PSB also showed lower count (12.4 x 105 cfu g-1). In combination treatment of Pseudomonas fluorescence with bio fertilizers Psuedomonas fluorescence showed higher count (18.7 x 105cfu g-1) but Rhizobium showed lower count (12.4 x 105 cfu g-1) in one combination. Whereas, in second combination, Pseudomonas fluorescence showed higher count (19.0 x 105cfu g-1) but PSB showed lower count (12.1 x 105 cfu g-1).
The adjuvants like bio friendly polymer and sugar syrup, between these biological seed coating with bio-friendly polymer showed more viability and long shelf life for all the four biological agents compared to farmer practice of sugar syrup and the effect was more prominent in single inoculation compared to combined inoculations. Bio-friendly polymer recorded sufficient number of cfu in all the biological agents even after six months after storage. Six month after storage Trichoderma viride, Pseudomonas fluorescence were recorded high number of colonies (1.5 x 105 cfu g seed-1 and 1.1 x 105 cfu g seed-1,
respectively) with bio-friendly polymer, which were sufficient for colonization to give protection to the young seedlings against soil borne pathogens.
With regard to the type of inoculation, single inoculation with Trichoderma viride or Pseudomonas fluorescence recorded more viability and the longer shelf life (1.8 x 105 cfu g seed-1 or 1.6 x 105 cfu g seed-1, respectively) compare to the combined inoculation of Trichoderma viride, Pseudomonas fluorescence with bio-fertilizers (0.6 x 105 cfu g seed-1 and 0.6 x 105 cfu g seed-1, respectively). Rhizobium showed longer shelf life in combined inoculation with Pseudomonas fluorescence (1.0 x 105 cfu g seed-1) when compared to Trichoderma viride (0.8 x 105 cfu g seed-1). Whereas, PSB recorded longer shelf life in combined inoculation with Trichoderma viride (1.5 x 105 cfu g seed-1) compare to Pseudomonas fluorescence (0.4 x 105 cfu g seed-1).
Biological seed coating of pigeon pea with Trichoderma viride and Pseudomonas fluorescence with bio-friendly polymer recorded good seed storability with high seed germination (73% and 75%, respectively), low seed moisture content (8.9 and 8.8, respectively), seedling length (16.9 cm and 13.9 cm, respectively) seedling dry weight (0.870 g and 0.880 g, respectively), seedling vigour index-1(1076.5 and 1040, respectively), seedling vigour index-2 (64 and 66, respectively) and low electrical conductivity (36.59 μmhos 50 seed-1and 28.36 μmhos 50 seed-1, respectively).
 
Date 2017-01-03T10:32:51Z
2017-01-03T10:32:51Z
2016
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94073
 
Language en
 
Relation D10010;
 
Format application/pdf
 
Publisher PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY RAJENDRANAGAR, HYDERABAD