Record Details

Detection and characterization of pigeonpea sterility mosaic virus (PPSMV) and its management

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Detection and characterization of pigeonpea sterility mosaic virus (PPSMV) and its management
 
Creator L, Manjunatha
 
Contributor H. K, Ramappa
 
Subject pigeonpeas, diseases, crops, planting, biological phenomena, sole cropping, genotypes, enzymes, sowing, land resources
 
Description Sterility mosaic disease is considered as the “green plague of
pigeonpea” caused by pigeonpea sterility mosaic virus (PPSMV) and the
virus is vectored by eriophyid mite, Aceria cajani ChannaBasavanna.
Survey results revealed the occurrence of sterility mosaic disease (SMD)
incidence in the range of zero to 100 per cent in Karnataka. Highest
average incidence of 59.10 and 42.00 per cent was observed in
Ramanagara district during kharif 2010 and 2011, respectively. PPSMV
was not detected in the cotyledons of the plant in DAC-ELISA confirming
the non-transmissibility through seeds. Bioassay/host range studies
PPSMV with sap inoculation was successfully transmitted to Pigeonpea,
Phaseolus vulgaris and Nicotiana benthamiana and also confirmed by
ELISA and RT-PCR. Electron microscopic observation, ELISA and RTPCR
detection showed the presence of PPSMV in diseased plants. Out of
754 pigeonpea genotypes evaluated against SMD during kharif 2010 and
2011, seven genotypes namely ICP 7035, BRG 3, ICPL 87091, IPA 8F,
IPA 15 F, GT 101 and JKM 189 were found resistant. Out of 35 SSR
primers screened against SMD only four primers showed polymorphism.
Ultramicrotomy of leaf samples of resistant cultivar ICP-7035 revealed
the maximum thickness of both upper and lower epidermis compared to
susceptible cultivars ICP-2376, ICP-8863 and TTB-7. The SMD resistant
lines were found to contain high total phenols, sugars, peroxidase,
phenylalanine ammonialyase and polyphenol oxidase compared to
susceptible varieties. Combination of wettable sulphur +propargite and
wettable sulphur, dicofol, fenazaquin were found effective in reducing the
mite population (100%), SMD incidence and increasing the grain yield
compared to control.
 
Date 2016-05-18T10:32:19Z
2016-05-18T10:32:19Z
2012-09-10
 
Type Thesis
 
Identifier Th-10371
http://krishikosh.egranth.ac.in/handle/1/66053
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru