Detection and characterization of pigeonpea sterility mosaic virus (PPSMV) and its management
KrishiKosh
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Title |
Detection and characterization of pigeonpea sterility mosaic virus (PPSMV) and its management
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Creator |
L, Manjunatha
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Contributor |
H. K, Ramappa
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Subject |
pigeonpeas, diseases, crops, planting, biological phenomena, sole cropping, genotypes, enzymes, sowing, land resources
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Description |
Sterility mosaic disease is considered as the “green plague of pigeonpea” caused by pigeonpea sterility mosaic virus (PPSMV) and the virus is vectored by eriophyid mite, Aceria cajani ChannaBasavanna. Survey results revealed the occurrence of sterility mosaic disease (SMD) incidence in the range of zero to 100 per cent in Karnataka. Highest average incidence of 59.10 and 42.00 per cent was observed in Ramanagara district during kharif 2010 and 2011, respectively. PPSMV was not detected in the cotyledons of the plant in DAC-ELISA confirming the non-transmissibility through seeds. Bioassay/host range studies PPSMV with sap inoculation was successfully transmitted to Pigeonpea, Phaseolus vulgaris and Nicotiana benthamiana and also confirmed by ELISA and RT-PCR. Electron microscopic observation, ELISA and RTPCR detection showed the presence of PPSMV in diseased plants. Out of 754 pigeonpea genotypes evaluated against SMD during kharif 2010 and 2011, seven genotypes namely ICP 7035, BRG 3, ICPL 87091, IPA 8F, IPA 15 F, GT 101 and JKM 189 were found resistant. Out of 35 SSR primers screened against SMD only four primers showed polymorphism. Ultramicrotomy of leaf samples of resistant cultivar ICP-7035 revealed the maximum thickness of both upper and lower epidermis compared to susceptible cultivars ICP-2376, ICP-8863 and TTB-7. The SMD resistant lines were found to contain high total phenols, sugars, peroxidase, phenylalanine ammonialyase and polyphenol oxidase compared to susceptible varieties. Combination of wettable sulphur +propargite and wettable sulphur, dicofol, fenazaquin were found effective in reducing the mite population (100%), SMD incidence and increasing the grain yield compared to control. |
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Date |
2016-05-18T10:32:19Z
2016-05-18T10:32:19Z 2012-09-10 |
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Type |
Thesis
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Identifier |
Th-10371
http://krishikosh.egranth.ac.in/handle/1/66053 |
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Language |
en
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Format |
application/pdf
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Publisher |
University of Agricultural Sciences, Bengaluru
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