GENETIC DIVERSITY STUDY OF TWO DIFFERENT SPECIES OF OIL PALM (Elaeis oleifera and Elaeis guineensis) USING RAPD AND SSR MARKERS
KrishiKosh
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Title |
GENETIC DIVERSITY STUDY OF TWO DIFFERENT SPECIES OF OIL PALM (Elaeis oleifera and Elaeis guineensis) USING RAPD AND SSR MARKERS
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Creator |
LAHA, SAMRAT
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Contributor |
PRANAB KUMAR, MANDAL
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Subject |
oils, dna, rapd, genetics, fruits, polymorphism, breeds (animals), planting, selection, elaeis guineensis
SPECIES, OIL PALM (Elaeis oleifera and Elaeis guineensis) |
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Description |
Oil palm (Elaeis sp.) is a perennial diploid (2n=32) monocotyledon belonging to the family Arecaceae and is by far the highest oil yielder per unit area. The present investigation was conducted on oil palm at Directorate of Oil Palm Research (DOPR), Pedavegi, Andhra Pradesh [formerly known as National Research Centre for Oil Palm (NRCOP)]. The materials under study comprised of 17 E. guineensis Oil Palms from two accessions (240D X 281D and 80D X 281D) which were indigenously developed at Palode, Kerala and 23 E. oleifera palms again belonging to two accessions (oleifera-I and oleifera-II). RAPD analyses for 10 selected random primers (screened out of 50 random primers) were used across the 40 palms. A total number of 39 reproducible bands were scored, 34 being polymorphic. Similarity matrix revealed similarity between individuals ranged from 0.44 to 1.00 and a mean similarity value of 0.73. Dendrogram based on similarity matrix, differentiated 40 Oil Palms into two clusters of oleifera and xiii guineensis each, at minimum coefficient range of 0.70-0.75 (approx.). A result of relative significance in RAPD analysis was that of primer OPP-8, where band OPP-8.6 differentiated Elaeis oleifera from Elaeis guineensis in presence and absence respectively. Such a primer could possibly be used (after further verifications with a larger number of palms) not only to differentiate the two species, but also to identify an inter-specific hybrid from Elaeis guineensis, RAPD being a dominant marker. In SSR analyses, 8 SSR primers were used across the 40 palms (selected from list of primers prepared by Sujatha G, 2009). A total no. of 11 reproducible bands was scored, 4 being polymorphic. Similarity matrix revealed similarity between individuals which ranged from 0.64 to 1.00 and with mean similarity value of 0.84, indicating less genetic diversity. Dendrogram based on similarity matrix, differentiated 40 Oil Palms into 5 clusters at minimum coefficient value of 1.00 (approx.). Scoring results of RAPD and SSR were combined and analysis was done. Similarity matrix revealed similarity between individuals which ranged from 0.64 to 0.98 and with mean similarity value of 0.75. Dendrogram based on similarity matrix, differentiated 40 Oil Palms into two clusters again of oleifera and guineensis each at minimum coefficient value of 0.79 (approx.). The clustering pattern of RAPD and SSR combined analysis was more similar to the clustering pattern of RAPD than SSR since more polymorphic markers were produced in case of RAPD. Therefore, this study confirmed that genetic diversity could successfully be assessed in perennial crops like oil palm in limited time span by using RAPD and SSR. Hence, this work has provided valuable information and showed some trend on genetic relation of important mother guineensis and oleifera palms, which can be used in oil palm breeding for intra and inter-specific hybridization programmes. |
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Date |
2016-06-06T10:36:05Z
2016-06-06T10:36:05Z 2010 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/66810
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Language |
en
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Relation |
D9050;
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Format |
application/pdf
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Publisher |
ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY
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