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GENETIC DIVERSITY STUDY OF TWO DIFFERENT SPECIES OF OIL PALM (Elaeis oleifera and Elaeis guineensis) USING RAPD AND SSR MARKERS

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Title GENETIC DIVERSITY STUDY OF TWO DIFFERENT SPECIES OF OIL PALM (Elaeis oleifera and Elaeis guineensis) USING RAPD AND SSR MARKERS
 
Creator LAHA, SAMRAT
 
Contributor PRANAB KUMAR, MANDAL
 
Subject oils, dna, rapd, genetics, fruits, polymorphism, breeds (animals), planting, selection, elaeis guineensis
SPECIES, OIL PALM (Elaeis oleifera and Elaeis guineensis)
 
Description Oil palm (Elaeis sp.) is a perennial diploid (2n=32) monocotyledon belonging to
the family Arecaceae and is by far the highest oil yielder per unit area. The present
investigation was conducted on oil palm at Directorate of Oil Palm Research (DOPR),
Pedavegi, Andhra Pradesh [formerly known as National Research Centre for Oil Palm
(NRCOP)]. The materials under study comprised of 17 E. guineensis Oil Palms from two
accessions (240D X 281D and 80D X 281D) which were indigenously developed at
Palode, Kerala and 23 E. oleifera palms again belonging to two accessions (oleifera-I
and oleifera-II).
RAPD analyses for 10 selected random primers (screened out of 50 random
primers) were used across the 40 palms. A total number of 39 reproducible bands
were scored, 34 being polymorphic. Similarity matrix revealed similarity between
individuals ranged from 0.44 to 1.00 and a mean similarity value of 0.73. Dendrogram
based on similarity matrix, differentiated 40 Oil Palms into two clusters of oleifera and
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guineensis each, at minimum coefficient range of 0.70-0.75 (approx.). A result of
relative significance in RAPD analysis was that of primer OPP-8, where band OPP-8.6
differentiated Elaeis oleifera from Elaeis guineensis in presence and absence
respectively. Such a primer could possibly be used (after further verifications with a
larger number of palms) not only to differentiate the two species, but also to identify
an inter-specific hybrid from Elaeis guineensis, RAPD being a dominant marker.
In SSR analyses, 8 SSR primers were used across the 40 palms (selected from
list of primers prepared by Sujatha G, 2009). A total no. of 11 reproducible bands was
scored, 4 being polymorphic. Similarity matrix revealed similarity between individuals
which ranged from 0.64 to 1.00 and with mean similarity value of 0.84, indicating less
genetic diversity. Dendrogram based on similarity matrix, differentiated 40 Oil Palms
into 5 clusters at minimum coefficient value of 1.00 (approx.).
Scoring results of RAPD and SSR were combined and analysis was done.
Similarity matrix revealed similarity between individuals which ranged from 0.64 to
0.98 and with mean similarity value of 0.75. Dendrogram based on similarity matrix,
differentiated 40 Oil Palms into two clusters again of oleifera and guineensis each at
minimum coefficient value of 0.79 (approx.). The clustering pattern of RAPD and SSR
combined analysis was more similar to the clustering pattern of RAPD than SSR since
more polymorphic markers were produced in case of RAPD.
Therefore, this study confirmed that genetic diversity could successfully be
assessed in perennial crops like oil palm in limited time span by using RAPD and SSR.
Hence, this work has provided valuable information and showed some trend on
genetic relation of important mother guineensis and oleifera palms, which can be used
in oil palm breeding for intra and inter-specific hybridization programmes.
 
Date 2016-06-06T10:36:05Z
2016-06-06T10:36:05Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66810
 
Language en
 
Relation D9050;
 
Format application/pdf
 
Publisher ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY