Studies on viral persistence in naturally infected animals with foot-and-mouth disease virus
KrishiKosh
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Title |
Studies on viral persistence in naturally infected animals with foot-and-mouth disease virus
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Creator |
Bora, Maloshrie
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Contributor |
Sharma, Ravindra
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Subject |
Foot-and-mouth disease virus (FMDV), Virus persistence, NSP-ELISA, Cytokines, OPF
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Description |
Foot-and-mouth disease virus (FMDV) is an important cause of an acute, systemic infection of cloven footed animals and often produces a persistent infection following clinical or sub clinical disease in either naïve or vaccinated ruminants. The present study was undertaken to study about the various mechanisms of viral persistence with an aim to improvise the carrier animal detection assays. The non structural protein ELISA (NSP-ELISA) was used to detect the antibodies against 3AB3 NSP of FMDV in the bovine serum samples from a total of 4200 randomly selected animals, 200 from each of the 21 district of Haryana. A rapid recombinant FMDV 3AB3 and 3A NSP antigen-based latex agglutination test (LAT) was developed to detect anti-FMDV NSP. Oesophageal pharyngeal fluid (OPF) and serum samples from FMDV infected and recovered animals were collected at 45, 90 and 150 days post infection (dpi) and different methods like virus isolation, Quantitative Reverse trnscription Polymerase Chain Reaction (qRT-PCR), Loop Mediated Isothermal Amplification (LAMP) and NSP-ELISA were employed for detection of carrier animals. qRT-PCR was done for relative quantification of cytokine mRNA (IL-2, IFN-γ and TNF-α) in OPF. Virus-specific IgA and salivary IgG was also detected in serum and OPF at different dpi. Of the 4200 samples, 1.809% serum samples were found positive for anti-3AB3 NSP antibodies by 3AB3 NSP-ELISA. r3AB3 and r3A antigen coated LAT was applied to test 2100 serum samples of bovines ; of which 8.76% and 18.9% serum samples were found positive for anti-3AB3 and anti 3A-NSP FMDV antibodies respectively. Out of the eleven OPF samples, LAMP assay detected FMDV in five samples as compared to three by qRT-PCR and two by virus isolation. All the 11 serum samples collected from FMD recovered animals were found to be positive for anti-3AB3 NSP FMDV antibody upto 150dpi. The mRNA expression levels of the cytokines revealed downregulation of mRNAs of IL-2, IFN-γ and TNF-α. All the animals showed significant level of detectable IgA and IgG response upto 150 dpi although the antibody titre in serum and OPF was highest in 45 dpi which decreased gradually. Titres of IgA and IgG were higher in serum than in OPF. IgG was found to be the predominant subclass of antibody both in serum and OPF. |
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Date |
2016-08-17T09:07:16Z
2016-08-17T09:07:16Z 2014 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/72676
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Language |
en
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Format |
application/pdf
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Publisher |
LUVAS
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