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Studies on viral persistence in naturally infected animals with foot-and-mouth disease virus

KrishiKosh

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Title Studies on viral persistence in naturally infected animals with foot-and-mouth disease virus
 
Creator Bora, Maloshrie
 
Contributor Sharma, Ravindra
 
Subject Foot-and-mouth disease virus (FMDV), Virus persistence, NSP-ELISA, Cytokines, OPF
 
Description Foot-and-mouth disease virus (FMDV) is an important cause of an acute, systemic infection
of cloven footed animals and often produces a persistent infection following clinical or sub clinical
disease in either naïve or vaccinated ruminants. The present study was undertaken to study about the
various mechanisms of viral persistence with an aim to improvise the carrier animal detection assays.
The non structural protein ELISA (NSP-ELISA) was used to detect the antibodies against
3AB3 NSP of FMDV in the bovine serum samples from a total of 4200 randomly selected animals, 200
from each of the 21 district of Haryana. A rapid recombinant FMDV 3AB3 and 3A NSP antigen-based
latex agglutination test (LAT) was developed to detect anti-FMDV NSP. Oesophageal pharyngeal fluid
(OPF) and serum samples from FMDV infected and recovered animals were collected at 45, 90 and
150 days post infection (dpi) and different methods like virus isolation, Quantitative Reverse
trnscription Polymerase Chain Reaction (qRT-PCR), Loop Mediated Isothermal Amplification
(LAMP) and NSP-ELISA were employed for detection of carrier animals. qRT-PCR was done for
relative quantification of cytokine mRNA (IL-2, IFN-γ and TNF-α) in OPF. Virus-specific IgA and
salivary IgG was also detected in serum and OPF at different dpi.
Of the 4200 samples, 1.809% serum samples were found positive for anti-3AB3 NSP
antibodies by 3AB3 NSP-ELISA. r3AB3 and r3A antigen coated LAT was applied to test 2100 serum
samples of bovines ; of which 8.76% and 18.9% serum samples were found positive for anti-3AB3 and
anti 3A-NSP FMDV antibodies respectively. Out of the eleven OPF samples, LAMP assay detected
FMDV in five samples as compared to three by qRT-PCR and two by virus isolation. All the 11 serum
samples collected from FMD recovered animals were found to be positive for anti-3AB3 NSP FMDV
antibody upto 150dpi. The mRNA expression levels of the cytokines revealed downregulation of
mRNAs of IL-2, IFN-γ and TNF-α. All the animals showed significant level of detectable IgA and IgG
response upto 150 dpi although the antibody titre in serum and OPF was highest in 45 dpi which
decreased gradually. Titres of IgA and IgG were higher in serum than in OPF. IgG was found to be the
predominant subclass of antibody both in serum and OPF.
 
Date 2016-08-17T09:07:16Z
2016-08-17T09:07:16Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/72676
 
Language en
 
Format application/pdf
 
Publisher LUVAS