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Evaluation of Canine Diets Based on In Vitro Hindgut Fermentation and Fecal Microbiota

KrishiKosh

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Title Evaluation of Canine Diets Based on In Vitro Hindgut Fermentation and Fecal Microbiota
 
Creator Madhusudhan, H. S.
 
Contributor CHANDRAPAL SINGH, K.
 
Subject fermentation, biological phenomena, productivity, bacteria, proteins, microbial flora, research methods, livestock, starch, sampling
 
Description This study was carried out to assess the possibility of using gas production technique to evaluate canine diet and to assess the changes in hindgut microbial population of dogs feddifferent diets. The experiment comprised of in vitro digestibility, hindgut fermentation and total gas measurement, and enumeration of fecal microbiota. Four adult maintenance type
canine diets viz. homemade vegetable (HV), homemade non vegetarian (HNV), commercial vegetable (CV) and commercial non vegetarian (CNV) were selected. In vitro digestibility was completed by incubating the food samples with enzymes (pepsin, amylase and pancreatin) to simulate digestion in the stomach and small intestine. The residue obtained from enzymatic digestion was subjected to in vitro gas test to simulate the hindgut fermentation. Fecal samples from dogs fed experimental diets were used as the source of inoculum for incubating the food samples in gas production test. The fecal samples obtained
from dogs fed different experimental diets were subjected for bacterial enumeration (Coliforms, Streptococci, Lactobacilli, Clostridia, Bifidobacteria, Bacteroides and Total anaerobes) by pour plate and spread plate methods. The in vitro digestibility (%) of DM, CP
and CF of CV (85.51, 89.68 and 69.63 respectively) and CNV diet 84.35, 89.74 and 69.16 respectively) were higher than HV (81.85, 81.20 and 13.65 respectively) and HNV diet (88.85, 86.15 and 46.43 respectively) (p
 
Date 2016-06-21T13:23:10Z
2016-06-21T13:23:10Z
2010-05-28
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67584
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar