Antigenic Profile And Immunodiagnosis Of Cystic Echinococcosis
KrishiKosh
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Title |
Antigenic Profile And Immunodiagnosis Of Cystic Echinococcosis
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Creator |
Thilakan, N. Jeya
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Contributor |
Basith, S. Abdul
John, Lalitha Chandran, Daniel Joy Raj, G. Dhinakar |
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Description |
The antigenic profile of various hydatid antigens viz., Hydatid cyst fluid antigen (HCFA), Protoscolices antigen (PSA) and Germinal membrane antigen (GMA) were prepared and purified by gel filtration chromatography. Characterisation by SDS-PAGE revealed 9 protein bands at 8, 16, 24, 38, 58, 68, 98, 116 and 205 kDa for HCFA, 3 protein bands at 29, 72 and 98 kDa for PSA and 4 protein bands at 29, 38, 127 and 134 kDa for GMA. Western blot analysis of these antigens revealed no cross reaction between them. Counter immuno electrophoresis (CIEP) and western blot tests were used to identify hydatid specific antigen. CIEP analysis of the 3 metacestode antigens revealed cross reactivity between them. Western blot analysis of fluid antigens of thesemetacestode antigens with anti HCFA serum recognized high molecular weight protein bands in all antigens and low molecular 8 kDa antigen in hydatid cyst fluid antigen only. Hence 8 kDa protein of HCFA was identified as non cross reactive hydatid specific, immuno diagnostic antigen. The non cross reactive hydatid specific immunodiagnostic 8 kDa antigen from hydatid cyst fluid was prepared by DEAE-Sepharose fast flow anion exchange chromatography. Cross reactive studies between 8 kDa Coenurus and Cysticercus was carried out by CIEP using anti 8 kDa hyper immune sera and found no cross reaction between them. Hence the 8 kDa antigen was used for standardization of sero diagnostic tests such as CIEP, Latex agglutination test (LAT), Enzyme linked immuno electro transfer blot (EITB) and Flow through technique (FTT). Evaluation of these tests were carried out with 150 cattle, 150 sheep hydatid positive sea, 150 cattle and 150 sheep hydatid negative sera, 50 suspected and 50 negative human sera. The sensitivity, specificity, positive predictive value, negative predictive value and efficacy in diagnosis of CE in sheep were 78.8, 93, 94.6, 74.6 and 84.6% for CIEP, 89, 98.5, 98.6, 88 and 93.3% for LAT, 92.5, 99.28, 99.3, 92 and 95.6% for EITB and 94.9, 99.3, 99.33, 94.6 and 97% for FTT respectively. The sensitivity, specificity, positive predictive value, negative predictive value and efficiency for cystic echinococcosis in cattle were 75, 89.6, 92, 69.3 and 80.6% for CIEP, 85.5, 94, 94.6, 84 and 89.3% for LAT, 87.8, 96.29, 96.6, 86.6 and 91.6% for EITB and 92.4, 97.18, 97.33, 92 and 94.66% for FTTrespectively. All the four tests equally detected 2 positive cases in man. However the sensitivity and efficiency of FTT was higher than other tests. EITB and FTT had equal specificity in detecting antibodies in cattle and sheep. The current study is a pioneering work on standardization and evaluation of four serological tests viz., CIEP, LAT, EITB and FTT in diagnosis of CE in animals and man using the hydatid specific purified 8 kDa antigen. Flow through technique which was not used in the diagnosis of hydatidosis was adopted and found to be a simple and sensitive test in diagnosis of CE in man and animals with easy applicability in field condition. A kit based on this test can be developed for diagnosis of CE in man and animals. |
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Date |
2016-05-27T16:31:44Z
2016-05-27T16:31:44Z 2007 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/66408
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Language |
en
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Format |
application/pdf
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Publisher |
Tamil Nadu Veterinary and Animal Sciences University
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