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Ultrasonographic studies on effect of hcg administration on follicular and luteal dynamics vis-à-vis progesterone profile and conception rate in cross-bred cows

KrishiKosh

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Title Ultrasonographic studies on effect of hcg administration on follicular and luteal dynamics vis-à-vis progesterone profile and conception rate in cross-bred cows
 
Creator Agarwal, Sanjay
 
Contributor Gupta, H.P.
 
Subject ultrasonograpy, HCG, follicular fluid, luteal cells, progesterone, conception rate, crossbreds, cows
 
Description Thesis-PhD
The present study was aimed to increase progesterone level endogenously by hCG (Chorulon®, 1500 I.U.) administration, on day 0 (Group 2), 7 (Group 3), 14 (Group 4) and on
all days 0,7 and 14 (Group 5) of the estrous cycle inducing formation of accessory corpus luteum, thus preventing early embryonic mortality due to possible luteal insufficiency, monitoring size of preovulatory follicle, subsequent corpus luteum, formation of accessory corpus luteum vis-à-vis progesterone profile and conception rate in cross-bred cows. Healthy and normal cyclic cross bred cows (n= 50) having 3 wave estrous cycle were used in this study. Animals with pre-ovulatory follicle of ≥ 10 mm were bred artificially. The plasma progesterone was estimated using RIA kit. Ultrasonography and blood collection was done on day 0, 1, 2, 7, 14, 20, 25, 30, 35, 40 and 60 of estrous cycle. Pregnancy diagnosis was confirmed at day 60 post breeding. Preovulatory follicle had the largest diameter among all the days of estrous cycle. In all the pregnant animals the regular wave like follicular growth occurred till last day of our observation (day 60). In non-pregnant cows hCG treatment resulted into lengthening of the estrous cycle in all groups. Diameter and area of the corpus luteum in pregnant cows increased continuously from day 7 to day 60. In non-pregnant cows the corpus luteum diameter and area increased till day 14 and then decreased on day 20. In non-pregnant cows of treated group lengthening of estrous cycle occurred. No accessory corpus luteum was formed in cross-bred cows of control group. The formation of accessory corpus luteum was found maximum in number in the group 3 and group 4 of estrous cycle in which 5 accessory corpora lutea were formed in each group. The diameter and area of accessory corpus luteum was lesser than the diameter and area of corpus luteum, respectively. In pregnant cows the plasma progesterone concentration increased continuously from day 0 to day 60. In non-pregnant cows it increased from day 0 to day 14 and then declined. There was and significant rise in progesterone concentration due to accessory corpus luteum formation. The conception rate were 50%, 60%, 60%, 70% and 50% in group 1, 2, 3, 4 and 5, respectively. In group 4 the conception rate was highest among all the treatment and control group. It was concluded that there was no significant effect of hCG administration on diameter of pre-ovulatory or dominant follicle. The diameter and area of corpus luteum increased post hCG administration. Whereas, lengthening of estrous cycle and persistence of corpus luteum in non- pregnant cows occurred. The accessory corpus luteum could be produced more effectively on day 14 of estrous cycle, which is lesser in size and persists for longer duration (up to day 60; our last observation) in pregnant animals. After hCG administration there was significant rise in progesterone concentration, may be due to accessory corpus luteum formation or stimulatory effect on existing corpus luteum. The higher conception rate (70%) was obtained in group 4 (hCG administration on day 14) compared to other groups.
 
Date 2016-09-28T15:12:08Z
2016-09-28T15:12:08Z
2014-11
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/79324
 
Language en
 
Format application/pdf
 
Publisher G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)