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ISOLATION AND MOLECULAR CHARACTERIZATION OF CELLULOLYTIC FUNGI USED FOR CONVERSION OF SUGARCANE BIOMASS FOR BIOETHANOL PRODUCTION

KrishiKosh

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Title ISOLATION AND MOLECULAR CHARACTERIZATION OF CELLULOLYTIC FUNGI USED FOR CONVERSION OF SUGARCANE BIOMASS FOR BIOETHANOL PRODUCTION
 
Creator CHETAN, A M
 
Contributor HARINIKUMAR, K M
 
Subject ---
 
Description One of the major alternatives to fossil fuels that received major attention is
bioethanol derived from biomass. The cellulosic material are potential sources
of ethanol. Cellulolytic fungi are capable of degrading cellulose to smaller sugar
components like glucose units. The aim of this research was to isolate and
screen fungi capable of producing cellulases and to convert pre-treated
lignocellulosic material to fermentable sugars for the production of ethanol
using Saccharomyces cerevisiae. The lignocellulosic material such as sugarcane
bagasse and sugarcane trash were used as substrates for ethanol production.
Fungi were isolated from soil and compost samples collected from various
regions. The pure cultures were screened for the ability to degrade cellulose.
The cellulolytic activity was determined by measuring the clearing zone created
by the fungi. The cultures were further characterized using five random
primers. The fungi capable to produce cellulases were identified as Aspergillus
niger, Aspergillus fumigatus, Trichoderma viride, Trichoderma harzianum and
Trichoderma reesei based on colony characters, microscopic observation and
identification at molecular level based on DNA coding for 18S rRNA. The
substrates were powdered and pretreated with fungal isolates using Mandels’
and Reese media. The substrates were used as a carbon source. Sugarcane
bagasse and trash treated with Trichoderma reesei have shown highest
concentration of reducing sugars of 45.95mg/g and 40.56mg/g respectively and
ethanol yield of 11.56g/l and 10.92g/l respectively. From this study the fungal
cultures having the potential to degrade cellulosic material were identified and
they can be used for bioethanol production from lignocellulosic wastes.
 
Date 2016-12-07T09:30:23Z
2016-12-07T09:30:23Z
2012-07-31
 
Type Thesis
 
Identifier Th-10249
http://krishikosh.egranth.ac.in/handle/1/89367
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore