ISOLATION AND MOLECULAR CHARACTERIZATION OF CELLULOLYTIC FUNGI USED FOR CONVERSION OF SUGARCANE BIOMASS FOR BIOETHANOL PRODUCTION
KrishiKosh
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Title |
ISOLATION AND MOLECULAR CHARACTERIZATION OF CELLULOLYTIC FUNGI USED FOR CONVERSION OF SUGARCANE BIOMASS FOR BIOETHANOL PRODUCTION
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Creator |
CHETAN, A M
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Contributor |
HARINIKUMAR, K M
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Subject |
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Description |
One of the major alternatives to fossil fuels that received major attention is bioethanol derived from biomass. The cellulosic material are potential sources of ethanol. Cellulolytic fungi are capable of degrading cellulose to smaller sugar components like glucose units. The aim of this research was to isolate and screen fungi capable of producing cellulases and to convert pre-treated lignocellulosic material to fermentable sugars for the production of ethanol using Saccharomyces cerevisiae. The lignocellulosic material such as sugarcane bagasse and sugarcane trash were used as substrates for ethanol production. Fungi were isolated from soil and compost samples collected from various regions. The pure cultures were screened for the ability to degrade cellulose. The cellulolytic activity was determined by measuring the clearing zone created by the fungi. The cultures were further characterized using five random primers. The fungi capable to produce cellulases were identified as Aspergillus niger, Aspergillus fumigatus, Trichoderma viride, Trichoderma harzianum and Trichoderma reesei based on colony characters, microscopic observation and identification at molecular level based on DNA coding for 18S rRNA. The substrates were powdered and pretreated with fungal isolates using Mandels’ and Reese media. The substrates were used as a carbon source. Sugarcane bagasse and trash treated with Trichoderma reesei have shown highest concentration of reducing sugars of 45.95mg/g and 40.56mg/g respectively and ethanol yield of 11.56g/l and 10.92g/l respectively. From this study the fungal cultures having the potential to degrade cellulosic material were identified and they can be used for bioethanol production from lignocellulosic wastes. |
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Date |
2016-12-07T09:30:23Z
2016-12-07T09:30:23Z 2012-07-31 |
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Type |
Thesis
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Identifier |
Th-10249
http://krishikosh.egranth.ac.in/handle/1/89367 |
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Language |
en
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Format |
application/pdf
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Publisher |
University of Agricultural Sciences GKVK, Bangalore
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